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About 30,030 results

Blocking of inflammatory heparan sulfate domains by specific antibodies is not protecti...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8699719
PloS One; van Gemst JJ, Passmann NJHG et. al.

Dec 24th, 2021 - Glomerulonephritis is an acquired serious glomerular disease, which involves the interplay of many factors such as cytokines, chemokines, inflammatory cells, and heparan sulfate (HS). We previously showed that blocking of inflammatory heparan sulfate domains on cultured glomerular endothelium by specific anti-HS single chain antibodies reduced polymorphonuclear cell (PMN) adhesion and chemokine...

Metabolic drug survey highlights cancer cell dependencies and vulnerabilities.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8671470
Nature Communications; Pemovska T, Bigenzahn JW et. al.

Dec 16th, 2021 - Interrogation of cellular metabolism with high-throughput screening approaches can unravel contextual biology and identify cancer-specific metabolic vulnerabilities. To systematically study the consequences of distinct metabolic perturbations, we assemble a comprehensive metabolic drug library (CeMM Library of Metabolic Drugs; CLIMET) covering 243 compounds. We, next, characterize it phenotypic...

Type-I collagen produced by distinct fibroblast lineages reveals specific function duri...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8664945
Nature Communications; Chen Y, Yang S et. al.

Dec 12th, 2021 - Type I collagen (Col1) is the most abundant protein in mammals. Col1 contributes to 90% of the total organic component of bone matrix. However, the precise cellular origin and functional contribution of Col1 in embryogenesis and bone formation remain unknown. Single-cell RNA-sequencing analysis identifies Fap+ cells and Fsp1+ cells as the major contributors of Col1 in the bone. We generate tran...

2-((1H-indol-3-yl)thio)-N-phenyl-acetamides: SARS-CoV-2 RNA-dependent RNA polymerase in...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8600920
Antiviral Research; Zhao J, Zhang G et. al.

Nov 22nd, 2021 - Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is the causative agent of Coronavirus Disease 2019 (COVID-19) pandemic. Despite intensive and global efforts to discover and develop novel antiviral therapies, only Remdesivir has been approved as a treatment for COVID-19. Therefore, effective antiviral therapeutics are still urgently needed to combat and halt the pandemic. Viral RNA-...

Circ_SPG11 plays contributing effects on IL-1β-induced chondrocyte apoptosis and ECM de...
https://doi.org/10.1016/j.clim.2021.108889
Clinical Immunology (Orlando, Fla.); Ouyang X, Ding Y et. al.

Nov 20th, 2021 - The dysregulation of circular RNA (circRNA) has been monitored in osteoarthritis (OA) cartilage, hinting that circRNA deregulation modulates OA progression. We thus aimed to unveil the role of circRNA spastic paraplegia 11 (circ_SPG11) in OA conditions. The upregulation of circ_SPG11 was observed in OA cartilage and IL-1β-treated chondrocytes. Knockdown of circ_SPG11 restored IL-1β-depleted cel...

Heterologous expression and purification of recombinant human protoporphyrinogen oxidas...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8601502
PloS One; Novakova Z, Khuntsaria D et. al.

Nov 19th, 2021 - Human protoporphyrinogen oxidase IX (hPPO) is an oxygen-dependent enzyme catalyzing the penultimate step in the heme biosynthesis pathway. Mutations in the enzyme are linked to variegate porphyria, an autosomal dominant metabolic disease. Here we investigated eukaryotic cells as alternative systems for heterologous expression of hPPO, as the use of a traditional bacterial-based system failed to...

Mimics of Allergy and Angioedema: Scombroid, Mast Cell Activation Disorders, and Heredi...
https://doi.org/10.1016/j.emc.2021.09.005
Emergency Medicine Clinics of North America; Thomas EG, Thomas DJ

Nov 17th, 2021 - Scombroid poisoning, systemic mastocytosis, and hereditary alpha tryptasemia all present with episodes that resemble allergic reactions. Knowledge regarding systemic mastocytosis and hereditary alpha tryptasemia is quickly evolving. Epidemiology, pathophysiology, and strategies to identify and diagnose are discussed. Evidence-based management in the emergency setting and beyond is also explored...

Glomerular Endothelial Cell-Derived microRNA-192 Regulates Nephronectin Expression in I...
https://doi.org/10.1681/ASN.2020121699
Journal of the American Society of Nephrology : JASN; Müller-Deile J, Sopel N et. al.

Oct 31st, 2021 - Autoantibodies binding to podocyte antigens cause idiopathic membranous glomerulonephritis (iMGN). However, it remains elusive how autoantibodies reach the subepithelial space because the glomerular filtration barrier (GFB) is size selective and almost impermeable for antibodies. Kidney biopsies from patients with iMGN, cell culture, zebrafish, and mouse models were used to investigate the role...

Characterization of PD-1/PD-L1 immune checkpoint expression in the pathogenesis of musc...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8556058
Medicine Hashimoto K, Nishimura S et. al.

Oct 30th, 2021 - Recent data suggest that programmed cell death -1 (PD-1) and programmed cell death ligand-1 (PD-L1) are involved in the pathogenesis of Langerhans cell histiocytoma (LCH); however, their contributions are not well established. Also, the involvement of PD-1/PD-L1 molecules in musculoskeletal LCH remains particularly unclear. The current study aims to characterize the involvement of PD-1/PD-L1 im...

Novel regulators of PrPC biosynthesis revealed by genome-wide RNA interference.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8575309
PLoS Pathogens; Heinzer D, Avar M et. al.

Oct 28th, 2021 - The cellular prion protein PrPC is necessary for prion replication, and its reduction greatly increases life expectancy in animal models of prion infection. Hence the factors controlling the levels of PrPC may represent therapeutic targets against human prion diseases. Here we performed an arrayed whole-transcriptome RNA interference screen to identify modulators of PrPC expression. We cultured...

Apolipoprotein E-Containing Lipoproteins and LRP1 Protect From NMDA-Induced Excitotoxic...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8556555
Investigative Ophthalmology & Visual Science; Hayashi H, Mori M et. al.

Oct 27th, 2021 - Optic nerve damage leads to impairment of visual functions. We previously demonstrated that apolipoprotein E-containing lipoproteins (E-LPs) protect retinal ganglion cells (RGCs) from degeneration in a glaucoma model of glutamate/aspartate transporter-deficient mice. This study aimed to determine whether E-LPs protect RGCs from N-methyl-D-aspartate (NMDA)-induced excitotoxicity, and to investig...

Therapeutic Exon Skipping Through a CRISPR-Guided Cytidine Deaminase Rescues Dystrophic...
https://doi.org/10.1161/CIRCULATIONAHA.121.054628
Circulation Li J, Wang K et. al.

Oct 27th, 2021 - Loss of dystrophin protein causes Duchenne muscular dystrophy (DMD), characterized by progressive degeneration of cardiac and skeletal muscles, and mortality in adolescence or young adulthood. Although cardiac failure has risen as the leading cause of mortality in patients with DMD, effective therapeutic interventions remain underdeveloped, in part, because of the lack of a suitable preclinical...

Predictors of neutralizing antibody response to BNT162b2 vaccination in allogeneic hema...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8542409
Journal of Hematology & Oncology; Canti L, Humblet-Baron S et. al.

Oct 26th, 2021 - Factors affecting response to SARS-CoV-2 mRNA vaccine in allogeneic hematopoietic stem cell transplantation (allo-HCT) recipients remain to be elucidated. Forty allo-HCT recipients were included in a study of immunization with BNT162b2 mRNA vaccine at days 0 and 21. Binding antibodies (Ab) to SARS-CoV-2 receptor binding domain (RBD) were assessed at days 0, 21, 28, and 49 while neutralizing Ab ...

Converging purinergic and immune signaling pathways drive IL-6 secretion by Fragile X c...
https://doi.org/10.1016/j.jneuroim.2021.577745
Journal of Neuroimmunology; Reynolds KE, Krasovska V et. al.

Oct 26th, 2021 - The symptoms of Fragile X syndrome (FXS) are driven in part by abnormal glial-mediated function. FXS astrocytes release elevated levels of immune-related factors interleukin-6 (IL-6) and tenascin C (TNC), and also demonstrate increased purinergic signaling, a pathway linked to signaling factor release. Here, in cortical astrocytes from the Fmr1 knockout (KO) FXS mouse model, purinergic agonism ...

Receptor-binding domain of SARS-CoV-2 spike protein efficiently inhibits SARS-CoV-2 inf...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8495392
International Journal of Biological Sciences; Shin HJ, Ku KB et. al.

Oct 22nd, 2021 - COVID-19, caused by a novel coronavirus, SARS-CoV-2, poses a serious global threat. It was first reported in 2019 in China and has now dramatically spread across the world. It is crucial to develop therapeutics to mitigate severe disease and viral spread. The receptor-binding domains (RBDs) in the spike protein of SARS-CoV and MERS-CoV have shown anti-viral activity in previous reports suggesti...

A class II MHC-targeted vaccine elicits immunity against SARS-CoV-2 and its variants.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8612213
Proceedings of the National Academy of Sciences of the Un... Pishesha N, Harmand TJ et. al.

Oct 17th, 2021 - The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in over 100 million infections and millions of deaths. Effective vaccines remain the best hope of curtailing SARS-CoV-2 transmission, morbidity, and mortality. The vaccines in current use require cold storage and sophisticated manufacturing capacity, which complicates their distribution, especially ...

Loss of Mitochondrial Ca2+ Uniporter Limits Inotropic Reserve and Provides Trigger and ...
https://doi.org/10.1161/CIRCULATIONAHA.121.053755
Circulation Bertero E, Nickel A et. al.

Oct 15th, 2021 - Barth syndrome (BTHS) is caused by mutations of the gene encoding tafazzin, which catalyzes maturation of mitochondrial cardiolipin and often manifests with systolic dysfunction during early infancy. Beyond the first months of life, BTHS cardiomyopathy typically transitions to a phenotype of diastolic dysfunction with preserved ejection fraction, blunted contractile reserve during exercise, and...

Germline determinants of humoral immune response to HPV-16 protect against oropharyngea...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8511029
Nature Communications; Ferreiro-Iglesias A, McKay JD et. al.

Oct 14th, 2021 - Although several oropharyngeal cancer (OPC) susceptibility loci have been identified, most previous studies lacked detailed information on human papillomavirus (HPV) status. We conduct a genome-wide analysis by HPV16 serology status in 4,002 oral cancer cases (OPC and oral cavity cancer (OCC)) and 5,256 controls. We detect four susceptibility loci pointing to a distinct genetic predisposition b...

Somatostatin analogue pasireotide (SOM230) inhibits catecholamine secretion in human ph...
https://doi.org/10.1016/j.canlet.2021.10.009
Cancer Letters; Streit L, Moog S et. al.

Oct 13th, 2021 - Increasingly common, neuroendocrine tumors (NETs) are regarded nowadays as neoplasms potentially causing debilitating symptoms and life-threatening medical conditions. Pheochromocytoma is a NET that develops from chromaffin cells of the adrenal medulla, and is responsible for an excessive secretion of catecholamines. Consequently, patients have an increased risk for clinical symptoms such as hy...

New insights into genetic characteristics between multiple myeloma and COVID-19: An int...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8652836
International Journal of Laboratory Hematology; Wang F, Liu R et. al.

Oct 9th, 2021 - Multiple myeloma (MM) is a hematological malignancy. Coronavirus disease 2019 (COVID-19) infection correlates with MM features. This study aimed to identify MM prognostic biomarkers with potential association with COVID-19. Differentially expressed genes (DEGs) in five MM data sets (GSE47552, GSE16558, GSE13591, GSE6477, and GSE39754) with the same expression trends were screened out. Functiona...