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About 251,245 results

Highly active engineered IgG3 antibodies against SARS-CoV-2.
https://doi.org/10.1073/pnas.2107249118
Proceedings of the National Academy of Sciences of the Un... Kallolimath S, Sun L et. al.

Oct 3rd, 2021 - Monoclonal antibodies (mAbs) that efficiently neutralize SARS-CoV-2 have been developed at an unprecedented speed. Notwithstanding, there is a vague understanding of the various Ab functions induced beyond antigen binding by the heavy-chain constant domain. To explore the diverse roles of Abs in SARS-CoV-2 immunity, we expressed a SARS-CoV-2 spike protein (SP) binding mAb (H4) in the four IgG s...

Dysregulated expression of circular RNAs serve as diagnostic and prognostic markers in ...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8483828
Medicine Liu F, Wu X et. al.

Oct 2nd, 2021 - Recent studies have reported a connection between non-coding RNAs such as circular RNAs (circRNAs) and the prognosis of various cancers. However, the mechanism of circRNA in ovarian cancer and cervical cancer has not been consistent. We evaluated the diagnostic and prognostic roles of circRNAs in ovarian and cervical cancer by meta-analysis. Pooled hazard ratios with 95% confidence intervals we...

Analysis of SARS-CoV-2 infection dynamic in vivo using reporter-expressing viruses.
https://doi.org/10.1073/pnas.2111593118
Proceedings of the National Academy of Sciences of the Un... Ye C, Chiem K et. al.

Sep 26th, 2021 - Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of the current COVID-19 pandemic, is one of the biggest threats to public health. However, the dynamic of SARS-CoV-2 infection remains poorly understood. Replication-competent recombinant viruses expressing reporter genes provide valuable tools to investigate viral infection. Low levels of reporter gene expressed ...

The LarB carboxylase/hydrolase forms a transient cysteinyl-pyridine intermediate during...
https://doi.org/10.1073/pnas.2106202118
Proceedings of the National Academy of Sciences of the Un... Rankin JA, Chatterjee S et. al.

Sep 23rd, 2021 - Enzymes possessing the nickel-pincer nucleotide (NPN) cofactor catalyze C2 racemization or epimerization reactions of α-hydroxyacid substrates. LarB initiates synthesis of the NPN cofactor from nicotinic acid adenine dinucleotide (NaAD) by performing dual reactions: pyridinium ring C5 carboxylation and phosphoanhydride hydrolysis. Here, we show that LarB uses carbon dioxide, not bicarbonate, as...

Retinal Degeneration Caused by Ago2 Disruption.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8447045
Investigative Ophthalmology & Visual Science; Chen XJ, Zhang CJ et. al.

Sep 17th, 2021 - Argonaute proteins are key players in small RNA-guided gene silencing processes. Ago2 is the member of the Argonaute subfamily with slicer endonuclease activity and is critical for microRNA homeostasis and indispensable for biological development. However, the impact of Ago2 dysregulation in the retina remains to be fully explored. In this study, we studied the role of Ago2 in mouse retina. We ...

The tangled history of mRNA vaccines.
https://doi.org/10.1038/d41586-021-02483-w 10.1073/pnas.86.16.6077 10.1038/274923a0 10.1038/274921a0 10.1093/nar/12.18.7035 10.1093/nar/12.18.7057 10.1126/science.1690918 10.1002/eji.1830230749 10.1126/science.1546298 10.1084/jem.184.2.465 10.1002/1521-4141(200001)30:1<1::AID-IMMU1>3.0.CO;2-# 10.1038/sj.gt.3302964 10.1016/S0005-2736(97)00238-1 10.1038/sj.gt.3300930 10.1074/jbc.M310175200 10.1016/j.immuni.2005.06.008 10.1016/j.stem.2010.08.012 10.1016/j.vaccine.2020.12.070 10.1007/s00508-021-01922-y 10.1007/s11095-004-1873-z 10.1073/pnas.1209367109
Nature Dolgin E

Sep 16th, 2021 - The tangled history of mRNA vaccines.|2021|Dolgin E,|economics,history,history,chemistry,genetics,immunology,therapy,history,metabolism,biosynthesis,genetics,history,history,chemistry,immunology,immunology,history,adverse effects,history,therapeutic use,

The RAD51 recombinase protects mitotic chromatin in human cells.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8433380
Nature Communications; Wassing IE, Graham E et. al.

Sep 12th, 2021 - The RAD51 recombinase plays critical roles in safeguarding genome integrity, which is fundamentally important for all living cells. While interphase functions of RAD51 in maintaining genome stability are well-characterised, its role in mitosis remains contentious. In this study, we show that RAD51 protects under-replicated DNA in mitotic human cells and, in this way, promotes mitotic DNA synthe...

Oncogenic KRAS is dependent upon an EFR3A-PI4KA signaling axis for potent tumorigenic a...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8429657
Nature Communications; Adhikari H, Kattan WE et. al.

Sep 11th, 2021 - The HRAS, NRAS, and KRAS genes are collectively mutated in a fifth of all human cancers. These mutations render RAS GTP-bound and active, constitutively binding effector proteins to promote signaling conducive to tumorigenic growth. To further elucidate how RAS oncoproteins signal, we mined RAS interactomes for potential vulnerabilities. Here we identify EFR3A, an adapter protein for the phosph...

Oxidative Stress Resistance 1 Gene Therapy Retards Neurodegeneration in the rd1 Mutant ...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8434758
Investigative Ophthalmology & Visual Science; Sahu B, Leon LM et. al.

Sep 11th, 2021 - Oxidative stress is a major factor underlying many neurodegenerative diseases. However, antioxidant therapy has had mixed results, possibly because of its indiscriminate activity. The purpose of our study was to determine if the human OXR1 (hOXR1) antioxidant regulatory gene could protect neurons from oxidative stress and delay photoreceptor cell death. The cone-like 661W cell line was transfec...

Bio-inspired poly-DL-serine materials resist the foreign-body response.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8423817
Nature Communications; Zhang D, Chen Q et. al.

Sep 9th, 2021 - Implantation-caused foreign-body response (FBR) is a commonly encountered issue and can result in failure of implants. The high L-serine content in low immunogenic silk sericin, and the high D-serine content as a neurotransmitter together inspire us to prepare poly-DL-serine (PSer) materials in mitigating the FBR. Here we report highly water soluble, biocompatible and easily accessible PSer hyd...

Deficiency of intestinal Bmal1 prevents obesity induced by high-fat feeding.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8423749
Nature Communications; Yu F, Wang Z et. al.

Sep 9th, 2021 - The role of intestine clock in energy homeostasis remains elusive. Here we show that mice with Bmal1 specifically deleted in the intestine (Bmal1iKO mice) have a normal phenotype on a chow diet. However, on a high-fat diet (HFD), Bmal1iKO mice are protected against development of obesity and related abnormalities such as hyperlipidemia and fatty livers. These metabolic phenotypes are attributed...

Genotype, Age, Genetic Background, and Sex Influence Epha2-Related Cataract Development...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8431977
Investigative Ophthalmology & Visual Science; Dave A, Craig JE et. al.

Sep 9th, 2021 - Age-related cataract is the leading cause of blindness worldwide. Variants in the EPHA2 gene increase the disease risk, and its knockout in mice causes cataract. We investigated whether age, sex, and genetic background, risk factors for age-related cataract, and Epha2 genotype influence Epha2-related cataract development in mice. Cataract development was monitored in Epha2+/+, Epha2+/-, and Eph...

Sense and Sense Ability in a Synthetic Genetic Code.
https://doi.org/10.1056/NEJMcibr2109352
The New England Journal of Medicine; Liu CC

Sep 9th, 2021 - Sense and Sense Ability in a Synthetic Genetic Code.|2021|Liu CC,|genetics,genetics,genetics,virology,biosynthesis,genetics,genetics,

Growth-coupled selection of synthetic modules to accelerate cell factory development.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421431
Nature Communications; Orsi E, Claassens NJ et. al.

Sep 8th, 2021 - Growth-coupled selection of synthetic modules to accelerate cell factory development.|2021|Orsi E,Claassens NJ,Nikel PI,Lindner SN,|biosynthesis,genetics,genetics,metabolism,methods,genetics,biosynthesis,genetics,genetics,metabolism,methods,

Nascent Ribo-Seq measures ribosomal loading time and reveals kinetic impact on ribosome...
https://doi.org/10.1038/s41592-021-01250-z 10.1002/wrna.1465 10.1016/j.ymeth.2009.04.020 10.1126/science.1168978 10.1038/nature10098 10.1371/journal.pone.0035044 10.1021/ja01028a027 10.1038/nmeth.4435 10.1038/nmeth.4582 10.1021/bi00836a016 10.1016/j.cell.2011.10.002 10.1093/nar/gkm1177 10.1093/nar/gkaa609 10.1111/febs.12786 10.1038/s41598-017-17618-1 10.5808/GI.2018.16.4.e28 10.1073/pnas.1006821107 10.1371/journal.pgen.1004692 10.1038/nbt.1861 10.1126/science.1215110 10.1038/nprot.2014.051 10.1093/nar/gkx1237 10.1091/mbc.e12-02-0135 10.1371/journal.pgen.1004368 10.1038/s41467-018-06184-3 10.1371/journal.pgen.0030146 10.1021/bi991187d 10.1371/journal.pone.0002532 10.1038/nmeth.3252 10.1002/dvdy.24183 10.1186/gb-2009-10-3-r25 10.1093/bioinformatics/btp352 10.1093/bioinformatics/btr509 10.1038/s41422-019-0168-1 10.1021/ac026117i 10.1038/nbt.1511 10.1021/pr101065j 10.1074/mcp.M113.031591 10.1038/nmeth.3901
Nature Methods; Schott J, Reitter S et. al.

Sep 5th, 2021 - In general, mRNAs are assumed to be loaded with ribosomes instantly upon entry into the cytoplasm. To measure ribosome density (RD) on nascent mRNA, we developed nascent Ribo-Seq by combining Ribo-Seq with progressive 4-thiouridine labeling. In mouse macrophages, we determined experimentally the lag between the appearance of nascent mRNA and its association with ribosomes, which was calculated ...

Efficacy and breadth of adjuvanted SARS-CoV-2 receptor-binding domain nanoparticle vacc...
https://doi.org/10.1073/pnas.2106433118
Proceedings of the National Academy of Sciences of the Un... King HAD, Joyce MG et. al.

Sep 3rd, 2021 - Emergence of novel variants of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) underscores the need for next-generation vaccines able to elicit broad and durable immunity. Here we report the evaluation of a ferritin nanoparticle vaccine displaying the receptor-binding domain of the SARS-CoV-2 spike protein (RFN) adjuvanted with Army Liposomal Formulation QS-21 (ALFQ). RFN vacci...

The polar oxy-metabolome reveals the 4-hydroxymandelate CoQ10 synthesis pathway.
https://doi.org/10.1038/s41586-021-03865-w 10.1093/braincomms/fcab002 10.1016/j.ajhg.2020.06.015 10.1038/s41436-020-01010-y 10.1093/brain/awab041 10.1038/s41422-019-0164-5 10.1074/jbc.M304982200 10.1126/science.aaw0112 10.1074/jbc.M115.688762 10.1016/j.abb.2004.08.015 10.1038/s42255-019-0105-0 10.1021/acs.biochem.5b01319 10.1016/j.bbapap.2014.01.001 10.1021/ja000076v 10.1016/S1074-5521(00)00043-0 10.1006/mgme.1999.2880 10.1021/bi035762w 10.1016/j.febslet.2006.05.018 10.1016/j.chembiol.2004.04.012 10.1128/jb.127.3.1108-1118.1976 10.1016/j.tibs.2017.06.008 10.1021/bi301674p 10.1074/jbc.M807579200 10.1016/j.chembiol.2016.08.008 10.1038/nbt.3683 10.1128/AEM.00701-20 10.1158/0008-5472.CAN-19-0650 10.1159/000362826 10.1038/s41586-020-2475-6 10.1194/jlr.M057919 10.1016/j.freeradbiomed.2017.06.006 10.1016/S0021-9258(19)76930-0 10.1038/nmicrobiol.2017.73 10.1038/ng.3984 10.1007/978-1-61779-080-5_8 10.1016/j.ymben.2016.12.010 10.1186/1471-2105-11-405 10.1002/(SICI)1096-9888(199603)31:3<255::AID-JMS290>3.0.CO;2-3 10.1126/scisignal.2004088 10.1158/2159-8290.CD-12-0095
Nature Banh RS, Kim ES et. al.

Sep 3rd, 2021 - Oxygen is critical for a multitude of metabolic processes that are essential for human life. Biological processes can be identified by treating cells with 18O2 or other isotopically labelled gases and systematically identifying biomolecules incorporating labeled atoms. Here we labelled cell lines of distinct tissue origins with 18O2 to identify the polar oxy-metabolome, defined as polar metabol...

Evaluation of the SARS-CoV-2 Antibody Response to the BNT162b2 Vaccine in Patients Unde...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8414193
JAMA Network Open; Yau K, Abe KT et. al.

Sep 3rd, 2021 - Patients undergoing hemodialysis have a high mortality rate associated with COVID-19, and this patient population often has a poor response to vaccinations. Randomized clinical trials for COVID-19 vaccines included few patients with kidney disease; therefore, vaccine immunogenicity is uncertain in this population. To evaluate the SARS-CoV-2 antibody response in patients undergoing chronic hemod...

AACC Practical Recommendations for Implementing and Interpreting SARS-CoV-2 Emergency U...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8083777
Clinical Chemistry; Zhang YV, Wiencek J et. al.

Sep 2nd, 2021 - The clinical laboratory continues to play a critical role in managing the coronavirus pandemic. Numerous US Food and Drug Administration emergency use authorization (EUA) and laboratory-developed test (LDT) serologic assays have become available. The performance characteristics of these assays and their clinical utility continue to be defined in real time during this pandemic. The AACC convened...

A guanidine-degrading enzyme controls genomic stability of ethylene-producing cyanobact...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8390497
Nature Communications; Wang B, Xu Y et. al.

Aug 28th, 2021 - Recent studies have revealed the prevalence and biological significance of guanidine metabolism in nature. However, the metabolic pathways used by microbes to degrade guanidine or mitigate its toxicity have not been widely studied. Here, via comparative proteomics and subsequent experimental validation, we demonstrate that Sll1077, previously annotated as an agmatinase enzyme in the model cyano...