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About 52,005 results

Anti-leishmanial physalins-Phytochemical investigation, in vitro evaluation against cli...
https://doi.org/10.1371/journal.pone.0274543
PloS One; Bano S, Bibi M et. al.

Nov 29th, 2022 - Cutaneous leishmaniasis (CL) is a major health problem in over 98 countries of the world, including Pakistan. The current treatments are associated with a number of adverse effects and availability problem of drugs. Therefore, there is an urgent need of easily available and cost effective treatments of CL- in Pakistan. The bioassay-guided fractionation and purification of crude extract of Physa...

Monkeypox-Associated Acute Kidney Injury and Foreseeable Impacts on Nephrology and Kidn...
https://doi.org/10.1159/000528041
Blood Purification; Reis T, de Assis Rocha Neves F et. al.

Nov 25th, 2022 - Monkeypox-Associated Acute Kidney Injury and Foreseeable Impacts on Nephrology and Kidney Transplantation Services.|2022|Reis T,de Assis Rocha Neves F,Fagundes A,See E,da Hora Passos R,|

Translatomic response of retinal Müller glia to acute and chronic stress.
https://doi.org/10.1016/j.nbd.2022.105931
Neurobiology of Disease; Chucair-Elliott AJ, Ocañas SR et. al.

Nov 25th, 2022 - Analysis of retina cell type-specific epigenetic and transcriptomic signatures is crucial to understanding the pathophysiology of retinal degenerations such as age-related macular degeneration (AMD) and delineating cell autonomous and cell-non-autonomous mechanisms. We have discovered that Aldh1l1 is specifically expressed in the major macroglia of the retina, Müller glia, and, unlike the brain...

Mycobacterial MenG: Partial Purification, Characterization, and Inhibition.
https://doi.org/10.1021/acsinfecdis.2c00190
ACS Infectious Diseases; Pujari V, Rozman K et. al.

Nov 24th, 2022 - Menaquinone (MK) is an essential component of the electron transport chain (ETC) in the gram-variable Mycobacterium tuberculosis and many Gram-positive pathogens. Three genes in the M. tuberculosis genome were annotated as methyltransferases involved in lipoquinone synthesis in mycobacteria. Heterologous expression of Rv0558 complemented an ubiE (the quinone C-methyltransferase involved in ubiq...

Synthetic nanobodies as tools to distinguish IgG Fc glycoforms.
https://doi.org/10.1073/pnas.2212658119
Proceedings of the National Academy of Sciences of the Un... Kao KS, Gupta A et. al.

Nov 22nd, 2022 - Protein glycosylation is a crucial mediator of biological functions and is tightly regulated in health and disease. However, interrogating complex protein glycoforms is challenging, as current lectin tools are limited by cross-reactivity while mass spectrometry typically requires biochemical purification and isolation of the target protein. Here, we describe a method to identify and characteriz...

KITD816V mutation in blood for the diagnostic screening of systemic mastocytosis and ma...
https://doi.org/10.1111/all.15584
Allergy Navarro-Navarro P, Álvarez-Twose I et. al.

Nov 18th, 2022 - Current diagnostic algorithms for systemic mastocytosis (SM) rely on the detection of KITD816V in blood to trigger subsequent bone marrow (BM) investigations. Here, we correlated the KITD816V mutational status of paired blood and BM samples from 368 adults diagnosed with mast cell activation syndrome (MCAS) and mastocytosis and determined the potential utility of investigating KITD816V in genom...

Myeloencephalitis as the only presentation of Omicron SARS-CoV-2 infection.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9668029
BMJ Case Reports; Dang TQ, La DT et. al.

Nov 16th, 2022 - SARS-CoV-2 is now a major global health issue and manifests mainly as a respiratory disorder. Several other complications involving hypercoagulability, cardiovascular system and central nervous system have been described in the literature. Among these atypical presentations, encephalopathy associated with SARS-CoV-2 is a rare entity with heterogenous clinical and radiological findings. The dire...

Blood transfusion in autoimmune rheumatic diseases.
https://doi.org/10.1016/j.transci.2022.103596
Transfusion and Apheresis Science : Official Journal of T... Goubran H, Ragab G et. al.

Nov 13th, 2022 - Autoimmune rheumatic disorders (ARD) represent a wide spectrum of disorders that affect in priority the joints, bones, muscles, and connective tissues. Examples of ARD include rheumatoid arthritis, systemic lupus erythematosus, Sjögren syndrome, polymyositis, systemic sclerosis, antiphospholipid syndrome and mixed connective tissue disease. Patients with ARD often require transfusion of red cel...

Ferrobotic swarms enable accessible and adaptable automated viral testing.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9645323
Nature Lin H, Yu W et. al.

Nov 10th, 2022 - Expanding our global testing capacity is critical to preventing and containing pandemics1-9. Accordingly, accessible and adaptable automated platforms that in decentralized settings perform nucleic acid amplification tests resource-efficiently are required10-14. Pooled testing can be extremely efficient if the pooling strategy is based on local viral prevalence15-20...

LncRNA PVT-1 promotes osteosarcoma cancer stem-like properties through direct interacti...
https://doi.org/10.1038/s41388-022-02538-w
Oncogene Tsang SV, Rainusso N et. al.

Nov 9th, 2022 - Osteosarcoma, the most common pediatric bone tumor, is an aggressive heterogeneous malignancy defined by complex chromosomal aberrations. Overall survival rates remain at ~70%, but patients with chemoresistant or metastatic disease have extremely poor outcomes of <30%. A subgroup of tumors harbor amplification of chromosome 8q24.2 and increased expression of the oncogenic long noncoding RNA (ln...

Apheresis and COVID-19 in intensive care unit (ICU).
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9624107
Transfusion and Apheresis Science : Official Journal of T... Xanthi Z, Vasiliki P et. al.

Nov 6th, 2022 - Coronavirus disease 2019 (COVID-19) is a contagious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The first known case was identified in Wuhan, China, in December 2019. The disease has since spread worldwide, and on March 2020 the World Health Organization (WHO) declared it as pandemic, causing a public health crisis. Symptoms of COVID-19 are variable, ranging ...

Anti-Inflammatory Approach in Chronic Dialysis Patients with SARS-CoV-2: ATA or PMMA Di...
https://doi.org/10.1159/000526319
Blood Purification; Donati G, Gasperoni L et. al.

Nov 3rd, 2022 - High-flux hemodialysis membranes may modulate the cytokine storm of SARS-CoV-2, but their impact on chronic hemodialysis (CHD) patients is unknown. The aim of the study was the evaluation of asymmetric cellulose triacetate (ATA) and polymethylmethacrylate (PMMA) dialyzers on inflammatory markers and clinical outcomes in CHD patients with SARS-CoV-2. A prospective, observational study on CHD pat...

The Sequential Use of Extracorporeal Cytokine Removal Devices in an Adolescent With COV...
https://doi.org/10.1097/MAT.0000000000001834
ASAIO Journal (American Society for Artificial Internal O... Hui WF, Chan RWY et. al.

Nov 2nd, 2022 - A 14-year-old male developed multisystem inflammatory syndrome in children (MIS-C) after acquiring the SARS-CoV-2 infection. He deteriorated rapidly requiring inotropic and ventilatory support as well as continuous renal replacement therapy (CRRT) due to rhabdomyolysis-associated acute kidney injury. A hemoadsoprtion column Cytosorb® was first incorporated into the CRRT circuit for myoglobin an...

Unplanned 30-Day Readmission Rates for Autosomal Dominant Polycystic Kidney Disease: In...
https://doi.org/10.1159/000526923
Blood Purification; Saha A, Ericksen P et. al.

Nov 2nd, 2022 - Among end-stage kidney disease (ESKD) patients on dialysis with autosomal dominant polycystic kidney disease (ADPKD), relatively little is known about the epidemiology and risk factors for 30-day readmissions in the USA. Therefore, we evaluated the 30-day unplanned readmission rates and predictors and inpatient care costs among ESKD patients with and without ADPKD using a nationally representat...

L-arginase from Streptomyces diastaticus MAM5 as a potential therapeutic agent in breas...
https://doi.org/10.1016/j.ijbiomac.2022.10.152
International Journal of Biological Macromolecules; Hassabo AA, Abdelraof M et. al.

Oct 28th, 2022 - Targeting cancer metabolic processes has increased interest over the last century. Cancer cells have an enhanced proliferation rate that requires high quantities of amino acids, including arginine. Therefore, arginine deprivation by L-arginase impairs tumor growth resulting in cell death. In the present study, L-arginine amidinohydrolase (L-arginase) from Streptomyces diastaticus was purified s...

Neuroinflammation Plays a Critical Role in Cerebral Cavernous Malformation Disease.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9669201
Circulation Research; Lai CC, Nelsen B et. al.

Oct 27th, 2022 - Cerebral cavernous malformations (CCMs) are neurovascular lesions caused by loss of function mutations in 1 of 3 genes, including KRIT1 (CCM1), CCM2, and PDCD10 (CCM3). CCMs affect ≈1 out of 200 children and adults, and no pharmacologic therapy is available. CCM lesion count, size, and aggressiveness vary widely among patients of similar ages with the same mutation or even within members of the...

Phage-Mediated Genetic Manipulation of the Lyme Disease Spirochete Borrelia burgdorferi.
https://doi.org/10.3791/64408
Journal of Visualized Experiments : JoVE; Eggers CH

Oct 25th, 2022 - Introducing foreign DNA into the spirochete Borrelia burgdorferi has been almost exclusively accomplished by transformation using electroporation. This process has notably lower efficiencies in the Lyme disease spirochete relative to other, better-characterized Gram-negative bacteria. The rate of success of transformation is highly dependent upon having concentrated amounts of high-quality DNA ...

A novel compound EPIC-0412 reverses temozolomide resistance via inhibiting DNA repair/M...
https://doi.org/10.1093/neuonc/noac242
Neuro-oncology Zhao J, Yang S et. al.

Oct 23rd, 2022 - TMZ resistance has become an important obstacle affecting its therapeutic benefits. O6-methylguanine DNA methyltransferase (MGMT) is primarily responsible for the TMZ resistance in GBM patients. Additionally, active DNA damage repair pathways can also lead to TMZ resistance. Here, we reported a novel small-molecule inhibitor EPIC-0412 that improved the therapeutic efficacy of TMZ by inhibiting ...

COVID-19 Study Undercuts 5-Day Isolation; Diet Doesn't Reduce Dementia Risk; and New Hope for Patients With Long COVID
https://www.medscape.com/viewarticle/982592

Oct 19th, 2022 - Why the 5-Day Isolation Period for COVID Makes No Sense New research suggests that the recommended 5-day isolation period for patients with COVID-19 is too short. Five days is the length of time recommended for isolation by the CDC, which cut that time from 10 days and eliminated a requirement for a negative antigen test to end isolation. Many individuals are symptomatic, and many people test p...

Modified expi293 cell culture system using piggyBac transposon enables efficient produc...
https://doi.org/10.1007/s12185-022-03468-9
International Journal of Hematology; Yoshimura T, Horiuchi K et. al.

Oct 14th, 2022 - Human blood coagulation factor VIII (hFVIII) is used in hemostatic and prophylactic treatment of patients with hemophilia A. Biotechnological innovations have enabled purification of the culture medium of rodent or human cells harboring the hFVIII expression cassette. However, cell lines express hFVIII protein derived from an exogenous expression vector at a lower level than most other proteins...