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About 8,175 results

Atomic Structures of Coxsackievirus B5 Provide Key Information on Viral Evolution and S...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9093117
Journal of Virology; Yang P, Shi D et. al.

Apr 21st, 2022 - Coxsackie virus B5 (CVB5), a main serotype in human Enterovirus B (EVB), can cause severe viral encephalitis and aseptic meningitis among infants and children. Currently, there is no approved vaccine or antiviral therapy available against CVB5 infection. Here, we determined the atomic structures of CVB5 in three forms: mature full (F) particle (2.73 Å), intermediate altered (A) particle (2.81 Å...

Chemical-genetic interaction mapping links carbon metabolism and cell wall structure to...
https://doi.org/10.1073/pnas.2201632119
Proceedings of the National Academy of Sciences of the Un... Koh EI, Oluoch PO et. al.

Apr 6th, 2022 - Current chemotherapy against Mycobacterium tuberculosis (Mtb), an important human pathogen, requires a multidrug regimen lasting several months. While efforts have been made to optimize therapy by exploiting drug–drug synergies, testing new drug combinations in relevant host environments remains arduous. In particular, host environments profoundly affect the bacterial metabolic state and drug e...

CD38 Expression by Circulating and Skin-Infiltrating Lymphocytes from Sezary Syndrome P...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8896155
Disease Markers; Quaglino P, Novelli M et. al.

Mar 8th, 2022 - Reports on the expression of CD38 in Sézary syndrome (SS), erythrodermic primary cutaneous T cell lymphoma with leukemic involvement, are limited. The aim of the present study is the analysis of the expression of CD38 by skin-infiltrating mononuclear cells and circulating T lymphocytes in a cohort of SS patients. SS patients diagnosed since 1985 in our clinic were retrospectively analyzed for C...

Evolution of the SARS-CoV-2 spike protein in the human host.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8897445
Nature Communications; Wrobel AG, Benton DJ et. al.

Mar 6th, 2022 - Recently emerged variants of SARS-CoV-2 contain in their surface spike glycoproteins multiple substitutions associated with increased transmission and resistance to neutralising antibodies. We have examined the structure and receptor binding properties of spike proteins from the B.1.1.7 (Alpha) and B.1.351 (Beta) variants to better understand the evolution of the virus in humans. Spikes of both...

Cryo-EM structure of a SARS-CoV-2 omicron spike protein ectodomain.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8894419
Nature Communications; Ye G, Liu B et. al.

Mar 5th, 2022 - The omicron variant of SARS-CoV-2 has been spreading rapidly across the globe. The virus-surface spike protein plays a critical role in the cell entry and immune evasion of SARS-CoV-2. Here we determined the 3.0 Å cryo-EM structure of the omicron spike protein ectodomain. In contrast to the original strain of SARS-CoV-2 where the receptor-binding domain (RBD) of the spike protein takes a mixtur...

Melanocytes and keratinocytes morphological changes in vitiligo patients. A histologica...
https://doi.org/10.1080/01913123.2022.2044946
Ultrastructural Pathology; Elsherif R, Mahmoud WA et. al.

Mar 5th, 2022 - Vitiligo is an idiopathic acquired chronic stigmatizing disease. It is a pigmentary disorder that affects the skin and the mucous membranes, and it is characterized by well-circumscribed, depigmented milky white macules and patches. It has an estimated prevalence of 0.5-2% of the population worldwide. In the previous studies, several mechanisms such as autoimmune, oxidative stress, genetic fact...

Homozygous SPAG6 variants can induce nonsyndromic asthenoteratozoospermia with severe M...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8886842
Reproductive Biology and Endocrinology : RB&E; Xu C, Tang D et. al.

Mar 3rd, 2022 - Multiple morphological abnormalities of the sperm flagella (MMAF) is a subtype of severe asthenoteratozoospermia with poorly understood genetic etiology. SPAG6 is a core axonemal component that plays a critical role in the formation of cilia and sperm flagella. Previous studies have reported that mutations in SPAG6 cause primary ciliary dyskinesia (PCD), but the association between SPAG6 gene v...

Mechanisms of Resistance to Noncovalent Bruton's Tyrosine Kinase Inhibitors.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9074143
The New England Journal of Medicine; Wang E, Mi X et. al.

Feb 24th, 2022 - Covalent (irreversible) Bruton's tyrosine kinase (BTK) inhibitors have transformed the treatment of multiple B-cell cancers, especially chronic lymphocytic leukemia (CLL). However, resistance can arise through multiple mechanisms, including acquired mutations in BTK at residue C481, the binding site of covalent BTK inhibitors. Noncovalent (reversible) BTK inhibitors overcome this mechanism and ...

Synthetic virions reveal fatty acid-coupled adaptive immunogenicity of SARS-CoV-2 spike...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8844029
Nature Communications; Staufer O, Gupta K et. al.

Feb 16th, 2022 - SARS-CoV-2 infection is a major global public health concern with incompletely understood pathogenesis. The SARS-CoV-2 spike (S) glycoprotein comprises a highly conserved free fatty acid binding pocket (FABP) with unknown function and evolutionary selection advantage1,2. Deciphering FABP impact on COVID-19 progression is challenged by the heterogenous nature and large molecular variability of l...

Differential activation mechanisms of lipid GPCRs by lysophosphatidic acid and sphingos...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8826421
Nature Communications; Liu S, Paknejad N et. al.

Feb 10th, 2022 - Lysophospholipids are bioactive lipids and can signal through G-protein-coupled receptors (GPCRs). The best studied lysophospholipids are lysophosphatidic acid (LPA) and sphingosine 1-phosphate (S1P). The mechanisms of lysophospholipid recognition by an active GPCR, and the activations of lysophospholipid GPCR-G-protein complexes remain unclear. Here we report single-particle cryo-EM structures...

Effect of ATP-sensitive Potassium Channel Openers on Intraocular Pressure in Ocular Hyp...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8822368
Investigative Ophthalmology & Visual Science; Roy Chowdhury U, Millar JC et. al.

Feb 8th, 2022 - To evaluate the effect of ATP-sensitive potassium channel openers cromakalim prodrug 1 (CKLP1) and diazoxide on IOP in three independent mouse models of ocular hypertension. Baseline IOP was measured in TGFβ2 overexpression, steroid-induced, and iris dispersion (DBA/2J) ocular hypertension mouse models, followed by once daily eyedrop administration with CKLP1 (5 mM) or diazoxide (5 mM). The IOP...

Evolution of TP53 abnormalities during CLL disease course is associated with telomere l...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8812042
BMC Cancer; Olbertova H, Plevova K et. al.

Feb 5th, 2022 - Telomeres are protective structures at chromosome ends which shorten gradually with increasing age. In chronic lymphocytic leukemia (CLL), short telomeres have been associated with unfavorable disease outcome, but the link between clonal evolution and telomere shortening remains unresolved. We investigated relative telomere length (RTL) in a well-characterized cohort of 198 CLL patients by qPCR...

Epigenetic clock analysis and increased plasminogen activator inhibitor-1 in high-funct...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8812940
PloS One; Okazaki S, Kimura R et. al.

Feb 4th, 2022 - Autism spectrum disorder (ASD) is characterized by impaired social communication and behavioral problems. An increased risk of premature mortality has been observed in individuals with ASD. Therefore, we hypothesized that biological aging is accelerated in individuals with ASD. Recently, several studies have established genome-wide DNA methylation (DNAm) profiles as 'epigenetic clocks' that can...

Myeloid bodies is not an uncommon ultrastructural finding.
https://doi.org/10.1080/01913123.2021.2022054
Ultrastructural Pathology; Choung HYG, Jean-Gilles J et. al.

Feb 2nd, 2022 - The presence of myeloid bodies (MBs) is classically associated with Fabry disease (FD). However, MBs are also identified in patients without clinical evidence of FD. We attempt to further understand the clinicopathologic significance of incidental MBs in those without FD. Among the 4400 renal biopsies accessioned at the University of Rochester Medical Center from 2010 to 2021, we identified 32 ...

The type IV pili component PilO is a virulence determinant of Francisella novicida.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8789160
PloS One; Ozanic M, Marecic V et. al.

Jan 26th, 2022 - Francisella tularensis is a highly pathogenic intracellular bacterium that causes the disease tularemia. While its ability to replicate within cells has been studied in much detail, the bacterium also encodes a less characterised type 4 pili (T4P) system. T4Ps are dynamic adhesive organelles identified as major virulence determinants in many human pathogens. In F. tularensis, the T4P is require...

Expansion microscopy of Plasmodium gametocytes reveals the molecular architecture of a ...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8789139
PLoS Pathogens; Rashpa R, Brochet M

Jan 26th, 2022 - Transmission of malaria-causing parasites to mosquitoes relies on the production of gametocyte stages and their development into gametes. These stages display various microtubule cytoskeletons and the architecture of the corresponding microtubule organisation centres (MTOC) remains elusive. Combining ultrastructure expansion microscopy (U-ExM) with bulk proteome labelling, we first reconstructe...

Interplay between an ATP-binding cassette F protein and the ribosome from Mycobacterium...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8782954
Nature Communications; Cui Z, Li X et. al.

Jan 23rd, 2022 - EttA, energy-dependent translational throttle A, is a ribosomal factor that gates ribosome entry into the translation elongation cycle. A detailed understanding of its mechanism of action is limited due to the lack of high-resolution structures along its ATPase cycle. Here we present the cryo-electron microscopy (cryo-EM) structures of EttA from Mycobacterium tuberculosis (Mtb), referred to as ...

Retinal pigment epithelium-specific CLIC4 mutant is a mouse model of dry age-related ma...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8766482
Nature Communications; Chuang JZ, Yang N et. al.

Jan 20th, 2022 - Age-related macular degeneration (AMD) is the leading cause of blindness among the elderly. Dry AMD has unclear etiology and no treatment. Lipid-rich drusen are the hallmark of dry AMD. An AMD mouse model and insights into drusenogenesis are keys to better understanding of this disease. Chloride intracellular channel 4 (CLIC4) is a pleomorphic protein regulating diverse biological functions. He...

Endothelial thrombomodulin downregulation caused by hypoxia contributes to severe infil...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8756077
EBioMedicine Won T, Wood MK et. al.

Jan 17th, 2022 - Thromboembolism is a life-threatening manifestation of coronavirus disease 2019 (COVID-19). We investigated a dysfunctional phenotype of vascular endothelial cells in the lungs during COVID-19. We obtained the lung specimens from the patients who died of COVID-19. The phenotype of endothelial cells and immune cells was examined by flow cytometry and immunohistochemistry (IHC) analysis. We teste...

A group of sclerosing epithelioid fibrosarcomas with low-level amplified EWSR1-CREB3L1 ...
https://doi.org/10.1016/j.prp.2021.153754
Pathology, Research and Practice; Zhang M, Yu Y et. al.

Jan 7th, 2022 - Sclerosing epithelioid fibrosarcoma (SEF), typically arising in middle-aged and older adults, is a rare malignant fibroblastic neoplasm characterized by epithelioid fibroblasts embedded in sclerotic hyalinized stroma. This tumor frequently harbors translocation between EWSR1 and CREB3 subfamily members. Here, we describe four cases of SEF with unique genetic characteristics in children. All tum...