×
About 253,335 results

Blocking of inflammatory heparan sulfate domains by specific antibodies is not protecti...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8699719
PloS One; van Gemst JJ, Passmann NJHG et. al.

Dec 24th, 2021 - Glomerulonephritis is an acquired serious glomerular disease, which involves the interplay of many factors such as cytokines, chemokines, inflammatory cells, and heparan sulfate (HS). We previously showed that blocking of inflammatory heparan sulfate domains on cultured glomerular endothelium by specific anti-HS single chain antibodies reduced polymorphonuclear cell (PMN) adhesion and chemokine...

Inhibition of β-catenin dependent WNT signalling upregulates the transcriptional repres...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8700011
PloS One; Haynes JM, Sibuea SM et. al.

Dec 24th, 2021 - In this study we investigate how β-catenin-dependent WNT signalling impacts midbrain dopaminergic neuron (mDA) specification. mDA cultures at day 65 of differentiation responded to 25 days of the tankyrase inhibitor XAV969 (XAV, 100nM) with reduced expression of markers of an A9 mDA phenotype (KCNJ6, ALDH1A1 and TH) but increased expression of the transcriptional repressors NR0B1 and NR0B2. Ove...

Improved bladder contractility after transplantation of human mesenchymal stem cells ov...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8694482
PloS One; Kim JH, Yang HJ et. al.

Dec 23rd, 2021 - An underactive bladder can lead to difficulty in voiding that causes incomplete emptying of the bladder, suggesting the need for a new strategy to increase bladder contractility in such patients. This study was performed to investigate whether human mesenchymal stem cells (hMSCs) were capable of restoring bladder contractility in rats with underactive bladder due to bladder outlet obstruction (...

Tobacco-free Nicotine - New Name, Same Scheme?
https://doi.org/10.1056/NEJMp2111159
The New England Journal of Medicine; Cwalina SN, McConnell R et. al.

Dec 18th, 2021 - Tobacco-free Nicotine - New Name, Same Scheme?|2021|Cwalina SN,McConnell R,Benowitz NL,Barrington-Trimis JL,|chemistry,biosynthesis,pharmacology,legislation & jurisprudence,

Combinations of slow-translating codon clusters can increase mRNA half-life in Saccharo...
https://doi.org/10.1073/pnas.2026362118
Proceedings of the National Academy of Sciences of the Un... Sharma AK, Venezian J et. al.

Dec 17th, 2021 - The presence of a single cluster of nonoptimal codons was found to decrease a transcript's half-life through the interaction of the ribosome-associated quality control machinery with stalled ribosomes in Saccharomyces cerevisiae The impact of multiple nonoptimal codon clusters on a transcript's half-life, however, is unknown. Using a kinetic model, we predict that inserting a second nonoptimal ...

Circular RNA circEsyt2 regulates vascular smooth muscle cell remodeling via splicing re...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8670847
The Journal of Clinical Investigation; Gong X, Tian M et. al.

Dec 16th, 2021 - Circular RNAs (circRNAs) have been recently recognized as playing a role in the pathogenesis of vascular remodeling-related diseases by modulating the functions of miRNAs. However, the interplay between circRNAs and proteins during vascular remodeling remains poorly understood. Here, we investigated a previously identified circRNA, circEsyt2, whose expression is known to be upregulated during v...

Metabolic drug survey highlights cancer cell dependencies and vulnerabilities.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8671470
Nature Communications; Pemovska T, Bigenzahn JW et. al.

Dec 16th, 2021 - Interrogation of cellular metabolism with high-throughput screening approaches can unravel contextual biology and identify cancer-specific metabolic vulnerabilities. To systematically study the consequences of distinct metabolic perturbations, we assemble a comprehensive metabolic drug library (CeMM Library of Metabolic Drugs; CLIMET) covering 243 compounds. We, next, characterize it phenotypic...

The Hippo Pathway Effectors YAP and TAZ Regulate LH Release by Pituitary Gonadotrope Ce...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8670590
Endocrinology Lalonde-Larue A, Boyer A et. al.

Dec 15th, 2021 - The Hippo transcriptional coactivators YAP and TAZ exert critical roles in morphogenesis, organ size determination and tumorigenesis in many tissues. Although Hippo kinase cascade activity was recently reported in the anterior pituitary gland in mice, the role of the Hippo effectors in regulating gonadotropin production remains unknown. The objective of this study was therefore to characterize ...

Platelet-Rich Plasma in Melasma-A Systematic Review.
https://doi.org/10.1097/DSS.0000000000003266
Dermatologic Surgery : Official Publication for American ... Sarkar R, Gupta M

Dec 15th, 2021 - Melasma is a common relapsing hyperpigmentation disorder, which is often difficult to treat. Platelet-rich plasma (PRP) is a novel modality often used to treat acne scars, androgenic alopecia, chronic wounds, and skin rejuvenation. Recently, it has had a promising role in the treatment of melasma. To review the published evidence on the efficacy and safety of PRP in the treatment of melasma. A ...

Complete biosynthesis of the bisbenzylisoquinoline alkaloids guattegaumerine and berbam...
https://doi.org/10.1073/pnas.2112520118
Proceedings of the National Academy of Sciences of the Un... Payne JT, Valentic TR et. al.

Dec 15th, 2021 - Benzylisoquinoline alkaloids (BIAs) are a diverse class of medicinal plant natural products. Nearly 500 dimeric bisbenzylisoquinoline alkaloids (bisBIAs), produced by the coupling of two BIA monomers, have been characterized and display a range of pharmacological properties, including anti-inflammatory, antitumor, and antiarrhythmic activities. In recent years, microbial platforms have been eng...

R3-MYB transcription factor LcMYBx from Litchi chinensis negatively regulates anthocyan...
https://doi.org/10.1016/j.gene.2021.146105
Gene Zhao J, Chen L et. al.

Dec 14th, 2021 - Anthocyanin accumulation is one of the remarkable physiological changes during fruit ripening. In plants, anthocyanin synthesis is regulated by MYB activators, but the MYB repressors has been recognized recently. Here, we isolated a repressor of anthocyanin synthesis, LcMYBx, from Litchi chinensis Sonn. LcMYBx encoded a typical R3-MYB protein and contained a conserved [D/E]Lx2[R/K]x3Lx6Lx3R mot...

Type-I collagen produced by distinct fibroblast lineages reveals specific function duri...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8664945
Nature Communications; Chen Y, Yang S et. al.

Dec 12th, 2021 - Type I collagen (Col1) is the most abundant protein in mammals. Col1 contributes to 90% of the total organic component of bone matrix. However, the precise cellular origin and functional contribution of Col1 in embryogenesis and bone formation remain unknown. Single-cell RNA-sequencing analysis identifies Fap+ cells and Fsp1+ cells as the major contributors of Col1 in the bone. We generate tran...

Molecular basis for redox control by the human cystine/glutamate antiporter system xc.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8654953
Nature Communications; Parker JL, Deme JC et. al.

Dec 10th, 2021 - Cysteine plays an essential role in cellular redox homoeostasis as a key constituent of the tripeptide glutathione (GSH). A rate limiting step in cellular GSH synthesis is the availability of cysteine. However, circulating cysteine exists in the blood as the oxidised di-peptide cystine, requiring specialised transport systems for its import into the cell. System xc- is a dedicated cystine trans...

Role of miR-181c in Diet-induced obesity through regulation of lipid synthesis in liver.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8654194
PloS One; Akiyoshi K, Boersma GJ et. al.

Dec 9th, 2021 - We recently identified a nuclear-encoded miRNA (miR-181c) in cardiomyocytes that can translocate into mitochondria to regulate mitochondrial gene mt-COX1 and influence obesity-induced cardiac dysfunction through the mitochondrial pathway. Because liver plays a pivotal role during obesity, we hypothesized that miR-181c might contribute to the pathophysiological complications associated with obes...

Enhancing the production of physiologically active vitamin D3 by engineering the hydrox...
https://doi.org/10.1007/s11274-021-03193-1 10.1080/14786419.2013.872104 10.1006/abbi.2000.1970 10.1046/j.1523-1747.2001.01332.x 10.1002/bmb.2004.494032060394 10.1042/bst0100147 10.1146/annurev.bi.52.070183.002211 10.1016/j.bbrc.2009.05.033 10.1016/j.bbapap.2009.07.012 10.1016/j.bbagen.2006.07.017 10.1021/bi801222d 10.1016/j.bbrc.2016.03.139 10.1006/abio.2001.5214 10.1016/j.bbrc.2011.01.038 10.1073/pnas.88.20.8972 10.1002/med.2610070202 10.1007/s00449-015-1368-9 10.1007/BF02932307 10.1016/0167-4781(94)90266-6 10.1016/j.procbio.2017.07.030 10.1080/10242422.2016.1268130 10.1002/bit.24632 10.1146/annurev.nu.11.070191.001201 10.1016/j.bbapap.2010.07.014 10.1128/aem.57.10.2841-2846.1991 10.1016/j.bbrc.2004.05.140 10.1021/bi7023767
World Journal of Microbiology & Biotechnology; Fu B, Ren Q et. al.

Dec 9th, 2021 - In this study, the conversion of vitamin D3 (VD3) to its two active forms 25(OH)VD3 and 1α, 25(OH)2VD3 was carried out by engineering the hydroxylase CYP105A1 and its redox partners Fdx and Fdr. CYP105A1 and Fdx-Fdr were respectively expressed in E. coli BL21(DE3) and purified. The electron transport chain Fdx-Fdr had higher selectivity for the coenzyme NADH than NADPH. HPLC analysis showed tha...

Gene excavation and expression analysis of CYP and UGT related to the post modifying st...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8651138
PloS One; Zhang Y, Chen Q et. al.

Dec 8th, 2021 - Previous studies have revealed that gypenosides produced from Gynostemma pentaphyllum (Thunb.) Makino are mainly dammarane-type triterpenoid saponins with diverse structures and important biological activities, but the mechanism of diversity for gypenoside biosynthesis is still unclear. In this study, a combination of isobaric tags for relative and absolute quantification (iTRAQ) proteome analy...

Reductive inactivation of the hemiaminal pharmacophore for resistance against tetrahydr...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8648761
Nature Communications; Wen WH, Zhang Y et. al.

Dec 8th, 2021 - Antibiotic resistance is becoming one of the major crises, among which hydrolysis reaction is widely employed by bacteria to destroy the reactive pharmacophore. Correspondingly, antibiotic producer has canonically co-evolved this approach with the biosynthetic capability for self-resistance. Here we discover a self-defense strategy featuring with reductive inactivation of hemiaminal pharmacopho...

Phenotypic effects from the expression of a deregulated AtGAD1 transgene and GABA pathw...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8648116
PloS One; S RM, Bedair MF et. al.

Dec 7th, 2021 - Glutamate decarboxylase (GAD; EC 4.1.1.15) catalyzes the irreversible decarboxylation of glutamate to produce γ-aminobutyric acid (GABA); a ubiquitous non-protein amino acid involved in the regulation of several aspects of plant metabolism and physiology. To study the function of GAD and GABA in maize, we have; 1) introduced native and deregulated forms of AtGAD1 into maize with the intent of i...

Downregulation of Dihydrotestosterone and Estradiol Levels by HEXIM1.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8645165
Endocrinology Mozar F, Sharma V et. al.

Dec 6th, 2021 - We have previously reported that hexamethylene bis-acetamide inducible protein 1 (HEXIM1) inhibits the activity of ligand-bound estrogen receptor α (ERα) and the androgen receptor (AR) by disrupting the interaction between these receptors and positive transcriptional elongation factor b (P-TEFb) and attenuating RNA polymerase II (RNAPII) phosphorylation at serine 2. Functional consequences of t...

High-intensity training induces non-stoichiometric changes in the mitochondrial proteom...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8642543
Nature Communications; Granata C, Caruana NJ et. al.

Dec 5th, 2021 - Mitochondrial defects are implicated in multiple diseases and aging. Exercise training is an accessible, inexpensive therapeutic intervention that can improve mitochondrial bioenergetics and quality of life. By combining multiple omics techniques with biochemical and in silico normalisation, we removed the bias arising from the training-induced increase in mitochondrial content to unearth an in...