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About 412,850 results

Expression of immune response genes in human corneal epithelial cells interacting with ...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8728928
BMC Genomics; Arunachalam D, Ramanathan SM et. al.

Jan 6th, 2022 - Aspergillus flavus, one of the causative agents of human fungal keratitis, can be phagocytosed by human corneal epithelial (HCE) cells and the conidia containing phagosomes mature into phagolysosomes. But the immunological responses of human corneal epithelial cells interacting with A. flavus are not clear. In this study, we report the expression of immune response related genes of HCE cells ex...

Identification of a distal RXFP1 gene enhancer with differential activity in fibrotic l...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8719731
PloS One; Chen TY, Li X et. al.

Jan 1st, 2022 - Relaxin/insulin-like family peptide receptor 1 (RXFP1) mediates relaxin's antifibrotic effects and has reduced expression in the lung and skin of patients with fibrotic interstitial lung disease (fILD) including idiopathic pulmonary fibrosis (IPF) and systemic sclerosis (SSc). This may explain the failure of relaxin-based anti-fibrotic treatments in SSc, but the regulatory mechanisms controllin...

Prolonged activation of nasal immune cell populations and development of tissue-residen...
https://doi.org/10.1038/s41590-021-01095-w 10.1038/s41586-020-2588-y 10.1016/j.xcrm.2020.100078 10.1126/science.abc6027 10.1038/s41590-021-01028-7 10.1038/s41591-020-0901-9 10.1038/s41587-020-0602-4 10.1016/j.immuni.2021.03.005 10.1016/j.cell.2021.07.023 10.1038/s41422-020-00455-9 10.1371/journal.pone.0169805 10.1182/blood-2016-04-713206 10.1016/j.immuni.2020.07.026 10.1189/jlb.0408256 10.15252/msb.20178124 10.1146/annurev.immunol.23.021704.115816 10.4049/jimmunol.182.3.1404 10.1016/j.immuni.2018.03.015 10.1016/j.cell.2020.05.015 10.1016/j.immuni.2020.10.006 10.1038/s41590-020-0782-6 10.1038/s41598-018-28791-2 10.1038/nprot.2015.020 10.1002/cyto.a.22826 10.3389/fimmu.2019.02367 10.1038/s41467-021-22811-y 10.4049/jimmunol.165.11.6229 10.1172/JCI86175 10.1038/nmeth.2639
Nature Immunology; Roukens AHE, Pothast CR et. al.

Dec 24th, 2021 - Systemic immune cell dynamics during coronavirus disease 2019 (COVID-19) are extensively documented, but these are less well studied in the (upper) respiratory tract, where severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) replicates1-6. Here, we characterized nasal and systemic immune cells in individuals with COVID-19 who were hospitalized or convalescent and compared the immune ce...

Direct visualization by FRET-FLIM of a putative mechanosome complex involving Src, Pyk2...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8699642
PloS One; Day RN, Day KH et. al.

Dec 24th, 2021 - Earlier, we proposed the "mechanosome" concept as a testable model for understanding how mechanical stimuli detected by cell surface adhesion molecules are transmitted to modulate gene expression inside cells. Here, for the first time we document a putative mechanosome involving Src, Pyk2 and MBD2 in MLO-Y4 osteocytes with high spatial resolution using FRET-FLIM. Src-Pyk2 complexes were concent...

Case 39-2021: A 26-Year-Old Woman with Respiratory Failure and Altered Mental Status.
https://doi.org/10.1056/NEJMcpc2107355
The New England Journal of Medicine; Kadar A, Shah VS et. al.

Dec 23rd, 2021 - Case 39-2021: A 26-Year-Old Woman with Respiratory Failure and Altered Mental Status.|2021|Kadar A,Shah VS,Mendoza DP,Lai PS,Aghajan Y,|diagnostic imaging,pathology,complications,diagnosis,cytology,complications,diagnosis,adverse effects,drug effects,pathology,

Lipid droplet availability affects neural stem/progenitor cell metabolism and prolifera...
https://doi.org/10.1038/s41467-021-27365-7
Nature Communications; Ramosaj M, Madsen S et. al.

Dec 23rd, 2021 - Neural stem/progenitor cells (NSPCs) generate new neurons throughout adulthood. However, the underlying regulatory processes are still not fully understood. Lipid metabolism plays an important role in regulating NSPC activity: build-up of lipids is crucial for NSPC proliferation, whereas break-down of lipids has been shown to regulate NSPC quiescence. Despite their central role for cellular lip...

Age-related changes in Kv4/Shal and Kv1/Shaker expression in Drosophila and a role for ...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8691634
PloS One; Vallejos MJ, Eadaim A et. al.

Dec 22nd, 2021 - Age-related changes in ion channel expression are likely to affect neuronal signaling. Here, we examine how age affects Kv4/Shal and Kv1/Shaker K+ channel protein levels in Drosophila. We show that Kv4/Shal protein levels decline sharply from 3 days to 10 days, then more gradually from 10 to 40 days after eclosion. In contrast, Kv1/Shaker protein exhibits a transient increase at 10 days that th...

MicroRNA-513c-5p is involved in the pathogenesis of preeclampsia by regulating of low-d...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8691017
BMC Pregnancy and Childbirth; Zhou Q, Li H et. al.

Dec 22nd, 2021 - Preeclampsia (PE) is a major cause of maternal and perinatal morbidity and mortality. Studies on the role of microRNAs (miRNAs), in the pathogenesis of PE through their effects on trophoblast function have been reported, but roles for some miRNAs including miR-513c-5p, have not been identified. We aimed to evaluate potential miRNA candidates that regulate the LRP6 mRNAand to elucidate the possi...

EpoR stimulates rapid cycling and larger red cells during mouse and human erythropoiesis.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8683474
Nature Communications; Hidalgo D, Bejder J et. al.

Dec 19th, 2021 - The erythroid terminal differentiation program couples sequential cell divisions with progressive reductions in cell size. The erythropoietin receptor (EpoR) is essential for erythroblast survival, but its other functions are not well characterized. Here we use Epor-/- mouse erythroblasts endowed with survival signaling to identify novel non-redundant EpoR functions. We find that, paradoxically...

N6-methyladenosine (m6A) depletion regulates pluripotency exit by activating signaling ...
https://doi.org/10.1073/pnas.2105192118
Proceedings of the National Academy of Sciences of the Un... Jin KX, Zuo R et. al.

Dec 19th, 2021 - N6-methyladenosine (m6A) deposition on messenger RNA (mRNA) controls embryonic stem cell (ESC) fate by regulating the mRNA stabilities of pluripotency and lineage transcription factors (TFs) [P. J. Batista et al., Cell Stem Cell 15, 707-719 (2014); Y. Wang et al., Nat. Cell Biol. 16, 191-198 (2014); and S. Geula et al., Science 347, 1002-1006 (2015)]. If the mRNAs of these two TF groups become ...

Induction of Rosette-to-Lumen stage embryoids using reprogramming paradigms in ESCs.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8677818
Nature Communications; Langkabel J, Horne A et. al.

Dec 18th, 2021 - Blastocyst-derived stem cell lines were shown to self-organize into embryo-like structures in 3D cell culture environments. Here, we provide evidence that embryo-like structures can be generated solely based on transcription factor-mediated reprogramming of embryonic stem cells in a simple 3D co-culture system. Embryonic stem cells in these cultures self-organize into elongated, compartmentaliz...

Place cells may simply be memory cells: Memory compression leads to spatial tuning and ...
https://doi.org/10.1073/pnas.2018422118
Proceedings of the National Academy of Sciences of the Un... Benna MK, Fusi S

Dec 18th, 2021 - The observation of place cells has suggested that the hippocampus plays a special role in encoding spatial information. However, place cell responses are modulated by several nonspatial variables and reported to be rather unstable. Here, we propose a memory model of the hippocampus that provides an interpretation of place cells consistent with these observations. We hypothesize that the hippoca...

EIN3 and RSL4 interfere with an MYB-bHLH-WD40 complex to mediate ethylene-induced ectop...
https://doi.org/10.1073/pnas.2110004118
Proceedings of the National Academy of Sciences of the Un... Qiu Y, Tao R et. al.

Dec 18th, 2021 - The alternating cell specifications of root epidermis to form hair cells or nonhair cells in Arabidopsis are determined by the expression level of GL2, which is activated by an MYB-bHLH-WD40 (WER-GL3-TTG1) transcriptional complex. The phytohormone ethylene (ET) has a unique effect of inducing N-position epidermal cells to form root hairs. However, the molecular mechanisms underlying ET-induced ...

Metformin attenuates osteoclast-mediated abnormal subchondral bone remodeling and allev...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8675877
PloS One; Guo H, Ding D et. al.

Dec 17th, 2021 - This study explored the mechanism by which metformin (Met) inhibits osteoclast activation and determined its effects on osteoarthritis (OA) mice. Bone marrow-derived macrophages were isolated. Osteoclastogenesis was detected using tartrate-resistant acid phosphatase (TRAP) staining. Cell proliferation was evaluated using CCK-8, F-actin rings were detected by immunofluorescence staining, and bon...

Bio-functionalization and in-vitro evaluation of titanium surface with recombinant fibr...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8675760
PloS One; Park BH, Jeong ES et. al.

Dec 17th, 2021 - Titanium is a biomaterial that meets a number of important requirements, including excellent mechanical and chemical properties, but has low bioactivity. To improve cellular response onto titanium surfaces and hence its osseointegration, the titanium surface was bio-functionalized to mimic an extracellular matrix (ECM)-like microenvironment that positively influences the behavior of stem cells....

Association between aqueous humor cytokines and postoperative corneal endothelial cell ...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8675763
PloS One; Okabe T, Kobayashi W et. al.

Dec 17th, 2021 - This study measured the intraoperative anterior aqueous humor concentrations of various cytokines during corneal endothelial transplantation and searched for relationships between these concentrations and postoperative corneal endothelial cell (CEC) depletion. We recruited 30 consecutive patients who underwent corneal endothelial transplantation with Descemet's stripping automated endothelial k...

Neuron-specific chromosomal megadomain organization is adaptive to recent retrotranspos...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8669064
Nature Communications; Chandrasekaran S, Espeso-Gil S et. al.

Dec 15th, 2021 - Regulatory mechanisms associated with repeat-rich sequences and chromosomal conformations in mature neurons remain unexplored. Here, we map cell-type specific chromatin domain organization in adult mouse cerebral cortex and report strong enrichment of Endogenous Retrovirus 2 (ERV2) repeat sequences in the neuron-specific heterochromatic B2NeuN+ megabase-scaling subcompartment. Single molecule l...

Cell-specific alterations in Pitx1 regulatory landscape activation caused by the loss o...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8668926
Nature Communications; Rouco R, Bompadre O et. al.

Dec 15th, 2021 - Developmental genes are frequently controlled by multiple enhancers sharing similar specificities. As a result, deletions of such regulatory elements have often failed to reveal their full function. Here, we use the Pitx1 testbed locus to characterize in detail the regulatory and cellular identity alterations following the deletion of one of its enhancers (Pen). By combining single cell transcr...

Constructing local cell-specific networks from single-cell data.
https://doi.org/10.1073/pnas.2113178118
Proceedings of the National Academy of Sciences of the Un... Wang X, Choi D et. al.

Dec 15th, 2021 - Gene coexpression networks yield critical insights into biological processes, and single-cell RNA sequencing provides an opportunity to target inquiries at the cellular level. However, due to the sparsity and heterogeneity of transcript counts, it is challenging to construct accurate gene networks. We develop an approach, locCSN, that estimates cell-specific networks (CSNs) for each cell, prese...

Theta activity paradoxically boosts gamma and ripple frequency sensitivity in prefronta...
https://doi.org/10.1073/pnas.2114549118
Proceedings of the National Academy of Sciences of the Un... Merino RM, Leon-Pinzon C et. al.

Dec 15th, 2021 - Fast oscillations in cortical circuits critically depend on GABAergic interneurons. Which interneuron types and populations can drive different cortical rhythms, however, remains unresolved and may depend on brain state. Here, we measured the sensitivity of different GABAergic interneurons in prefrontal cortex under conditions mimicking distinct brain states. While fast-spiking neurons always e...