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About 214,700 results

Effects of endurance training on the expression of host proteins involved in SARS-CoV-2...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8440939
Physiological Reports; Tamura Y, Jee E et. al.

Sep 16th, 2021 - The coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is threatening people's lives and impacting their health. It is still unclear whether people engaged in physical activity are at an increased risk of SARS-CoV-2 infection and severe forms of COVID-19. In order to provide data to help answer this question, we, therefore, inve...

The cryo-EM structure of the bd oxidase from M. tuberculosis reveals a unique structura...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8413341
Nature Communications; Safarian S, Opel-Reading HK et. al.

Sep 4th, 2021 - New drugs are urgently needed to combat the global TB epidemic. Targeting simultaneously multiple respiratory enzyme complexes of Mycobacterium tuberculosis is regarded as one of the most effective treatment options to shorten drug administration regimes, and reduce the opportunity for the emergence of drug resistance. During infection and proliferation, the cytochrome bd oxidase plays a crucia...

Peroxisome inspired hybrid enzyme nanogels for chemodynamic and photodynamic therapy.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8413279
Nature Communications; Qin X, Wu C et. al.

Sep 4th, 2021 - Peroxisome, a special cytoplasmic organelle, possesses one or more kinds of oxidases for hydrogen peroxide (H2O2) production and catalase for H2O2 degradation, which serves as an intracellular H2O2 regulator to degrade toxic peroxides to water. Inspired by this biochemical pathway, we demonstrate the reactive oxygen species (ROS) induced tumor therapy by integrating lactate oxidase (LOx) and ca...

RecA finds homologous DNA by reduced dimensionality search.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8443446
Nature Wiktor J, Gynnå AH et. al.

Sep 3rd, 2021 - Homologous recombination is essential for the accurate repair of double-stranded DNA breaks (DSBs)1. Initially, the RecBCD complex2 resects the ends of the DSB into 3' single-stranded DNA on which a RecA filament assembles3. Next, the filament locates the homologous repair template on the sister chromosome4. Here we directly visualize the repair of DSBs in single cells, using high-throughput mi...

Protein Phosphatase 4 Negatively Regulates the Immune Deficiency-NF-κB Pathway during t...
https://doi.org/10.4049/jimmunol.1901497
Journal of Immunology (Baltimore, Md. : 1950); Salem Wehbe L, Barakat D et. al.

Aug 29th, 2021 - The evolutionarily conserved immune deficiency (IMD) signaling pathway shields Drosophila against bacterial infections. It regulates the expression of antimicrobial peptides encoding genes through the activation of the NF-κB transcription factor Relish. Tight regulation of the signaling cascade ensures a balanced immune response, which is otherwise highly harmful. Several phosphorylation events...

A guanidine-degrading enzyme controls genomic stability of ethylene-producing cyanobact...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8390497
Nature Communications; Wang B, Xu Y et. al.

Aug 28th, 2021 - Recent studies have revealed the prevalence and biological significance of guanidine metabolism in nature. However, the metabolic pathways used by microbes to degrade guanidine or mitigate its toxicity have not been widely studied. Here, via comparative proteomics and subsequent experimental validation, we demonstrate that Sll1077, previously annotated as an agmatinase enzyme in the model cyano...

PTENα functions as an immune suppressor and promotes immune resistance in PTEN-mutant c...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8390757
Nature Communications; Sun Y, Lu D et. al.

Aug 28th, 2021 - PTEN is frequently mutated in human cancers and PTEN mutants promote tumor progression and metastasis. PTEN mutations have been implicated in immune regulation, however, the underlying mechanism is largely unknown. Here, we report that PTENα, the isoform of PTEN, remains active in cancer bearing stop-gained PTEN mutations. Through counteraction of CD8+ T cell-mediated cytotoxicity, PTENα leads ...

Paradoxical effects of cigarette smoke and COPD on SARS-CoV-2 infection and disease.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8381712
BMC Pulmonary Medicine; Tomchaney M, Contoli M et. al.

Aug 25th, 2021 - How cigarette smoke (CS) and chronic obstructive pulmonary disease (COPD) affect severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) infection and severity is controversial. We investigated the effects of COPD and CS on the expression of SARS-CoV-2 entry receptor ACE2 in vivo in COPD patients and controls and in CS-exposed mice, and the effects of CS on SARS-CoV-2 infection in human bro...

Proautoimmune Allele of Tyrosine Phosphatase, PTPN22, Enhances Tumor Immunity.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8428750
Journal of Immunology (Baltimore, Md. : 1950); Orozco RC, Marquardt K et. al.

Aug 22nd, 2021 - The 1858C>T allele of the tyrosine phosphatase PTPN22 (causing amino acid substitution R620W in encoded protein lymphoid tyrosine phosphatase) is present in 5-10% of the North American population and is strongly associated with numerous autoimmune diseases. Although much research has been done to define how this allele potentiates autoimmunity, the influence PTPN22 and its proautoimmune allele ...

Defining the therapeutic selective dependencies for distinct subtypes of PI3K pathway-a...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379232
Nature Communications; Mao N, Zhang Z et. al.

Aug 22nd, 2021 - Previous studies have suggested that PTEN loss is associated with p110β signaling dependency, leading to the clinical development of p110β-selective inhibitors. Here we use a panel pre-clinical models to reveal that PI3K isoform dependency is not governed by loss of PTEN and is impacted by feedback inhibition and concurrent PIK3CA/PIK3CB alterations. Furthermore, while pan-PI3K inhibition in PT...

Hypoxia alters the expression of ACE2 and TMPRSS2 SARS-CoV-2 cell entry mediators in hC...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8372440
Microvascular Research; Imperio GE, Lye P et. al.

Aug 21st, 2021 - The mechanisms by which the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) induces neurological complications remain to be elucidated. We aimed to identify possible effects of hypoxia on the expression of SARS-CoV-2 cell entry mediators, angiotensin-converting enzyme 2 (ACE2) receptor and transmembrane protease serine 2 (TMPRSS2) protein, in human brain endothelial cells, in vitro...

ROCK2, a critical regulator of immune modulation and fibrosis has emerged as a therapeu...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8456981
Clinical Immunology (Orlando, Fla.); Zanin-Zhorov A, Blazar BR

Aug 18th, 2021 - Chronic graft-versus-host disease (cGVHD) is an immune-mediated disorder characterized by chronic inflammation and fibrosis. Rho-associated coiled-coil-containing protein kinases (ROCKs) are key coordinators of tissue response to injury, regulating multiple functions, such as gene expression and cell migration, proliferation and survival. Relevant to cGVHD and autoimmunity, only the ROCK2 isofo...

To evaluate the serum cortisol, salivary cortisol, and serum interleukin-1 B level in p...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8341332
Medicine Zhang H, Chen B et. al.

Aug 17th, 2021 - The role of cognitive, social and biological factors in the etiology of chronic periodontitis has been reported.The aim of this study was to evaluate the salivary cortisol level and interleukin-1 B level in patients of Chronic periodontitis in smokers and stress and nonsmokers without stress.The design of study randomized, prospective, double-blinded, and prospective study.The total sample size...

Structure, mechanism and crystallographic fragment screening of the SARS-CoV-2 NSP13 he...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8358061
Nature Communications; Newman JA, Douangamath A et. al.

Aug 13th, 2021 - There is currently a lack of effective drugs to treat people infected with SARS-CoV-2, the cause of the global COVID-19 pandemic. The SARS-CoV-2 Non-structural protein 13 (NSP13) has been identified as a target for anti-virals due to its high sequence conservation and essential role in viral replication. Structural analysis reveals two "druggable" pockets on NSP13 that are among the most conser...

Timing the evolution of antioxidant enzymes in cyanobacteria.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8346466
Nature Communications; Boden JS, Konhauser KO et. al.

Aug 8th, 2021 - The ancestors of cyanobacteria generated Earth's first biogenic molecular oxygen, but how they dealt with oxidative stress remains unconstrained. Here we investigate when superoxide dismutase enzymes (SODs) capable of removing superoxide free radicals evolved and estimate when Cyanobacteria originated. Our Bayesian molecular clocks, calibrated with microfossils, predict that stem Cyanobacteria ...

Structural insights into how Prp5 proofreads the pre-mRNA branch site.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8357632
Nature Zhang Z, Rigo N et. al.

Aug 6th, 2021 - During the splicing of introns from precursor messenger RNAs (pre-mRNAs), the U2 small nuclear ribonucleoprotein (snRNP) must undergo stable integration into the spliceosomal A complex-a poorly understood, multistep process that is facilitated by the DEAD-box helicase Prp5 (refs. 1-4). During this process, the U2 small nuclear RNA (snRNA) forms an RNA duplex with the pre-mRNA branch site (the U...

Microbes exploit death-induced nutrient release by gut epithelial cells.
https://doi.org/10.1038/s41586-021-03785-9 10.1038/s41580-020-0232-1 10.1038/s41577-019-0240-6 10.1038/nature18849 10.1038/nature18845 10.1111/imr.12289 10.1038/nature18848 10.1016/j.cell.2018.04.036 10.1007/s00018-016-2205-2 10.1016/j.immuni.2020.04.002 10.1002/(SICI)1096-9896(199610)180:2<152::AID-PATH649>3.0.CO;2-Y 10.2147/JIR.S116088 10.1002/cncr.11671 10.1016/j.tibtech.2015.06.011 10.1038/s41586-020-2121-3 10.1038/s41467-018-07386-5 10.3389/fcimb.2020.00237 10.4049/jimmunol.1900072 10.1128/JB.00619-08 10.1093/cid/ciu1173 10.1016/S0016-5085(98)70019-8 10.1136/gutjnl-2013-305257 10.1038/nature22972 10.1111/j.1365-2958.1994.tb01262.x 10.1038/onc.2011.137 10.1038/nature09413 10.1128/IAI.71.5.2839-2858.2003 10.1111/mmi.13439 10.1046/j.1365-2958.1996.471410.x 10.1128/IAI.68.10.5702-5709.2000 10.1038/nature09415 10.1016/j.chom.2014.07.001 10.1016/j.chom.2014.07.002 10.1016/j.chom.2020.06.001 10.1016/j.ajpath.2012.06.016 10.1084/jem.20092474 10.1006/mpat.1993.1021 10.1111/apt.13302 10.1080/19490976.2016.1215806 10.1074/jbc.M400985200 10.1073/pnas.120163297 10.1128/AEM.01346-16 10.1371/journal.ppat.1003267 10.1371/journal.ppat.1005278 10.1128/mBio.01429-18 10.1038/s41467-019-09690-0 10.1038/nature13147 10.1371/journal.ppat.1007709
Nature Anderson CJ, Medina CB et. al.

Aug 6th, 2021 - Regulated cell death is an integral part of life, and has broad effects on organism development and homeostasis1. Malfunctions within the regulated cell death process, including the clearance of dying cells, can manifest in diverse pathologies throughout various tissues including the gastrointestinal tract2. A long appreciated, yet elusively defined relationship exists between cell death and ga...

RNA-dependent protein kinase is required for interferon-γ-induced autophagy in MG63 ost...
https://doi.org/10.1016/j.gene.2021.145865
Gene Xu J, Ji Y et. al.

Aug 6th, 2021 - Osteosarcoma is a bone tumor that mainly affects children and adolescents. Interferons (IFNs) have been shown to exert antitumor effects in osteosarcoma cells, although the molecular mechanisms have not been fully realized. We investigated IFN-γ actions on osteosarcoma cells. Our results show that IFN-γ induces the accumulation of autophagosomes in osteosarcoma cells. IFN-γ treatment leads to t...

The six steps of the complete F1-ATPase rotary catalytic cycle.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8333055
Nature Communications; Sobti M, Ueno H et. al.

Aug 5th, 2021 - F1Fo ATP synthase interchanges phosphate transfer energy and proton motive force via a rotary catalysis mechanism. Isolated F1-ATPase catalytic cores can hydrolyze ATP, passing through six intermediate conformational states to generate rotation of their central γ-subunit. Although previous structural studies have contributed greatly to understanding rotary catalysis in the F1-ATPase, the struct...

Flavonoids in Ampelopsis grossedentata as covalent inhibitors of SARS-CoV-2 3CLpro: Inh...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8322037
International Journal of Biological Macromolecules; Xiong Y, Zhu GH et. al.

Aug 2nd, 2021 - Coronavirus 3C-like protease (3CLpro) is a crucial target for treating coronavirus diseases including COVID-19. Our preliminary screening showed that Ampelopsis grossedentata extract (AGE) displayed potent SARS-CoV-2-3CLpro inhibitory activity, but the key constituents with SARS-CoV-2-3CLpro inhibitory effect and their mechanisms were unrevealed. Herein, a practical strategy via integrating bio...