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About 217,180 results

Axonal Protection by Netarsudil, a ROCK Inhibitor, Is Linked to an AMPK-Autophagy Pathw...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8742515
Investigative Ophthalmology & Visual Science; Kitaoka Y, Sase K et. al.

Jan 5th, 2022 - Netarsudil, a Rho kinase inhibitor with norepinephrine transport inhibitory effect, lowers intraocular pressure, however, its effect on axon damage remains to be elucidated. The aim of the current study was to investigate the effect of netarsudil on TNF-induced axon loss and to examine whether it affects phosphorylated-AMP-activated kinase (p-AMPK) and autophagy in the optic nerve. Intravitreal...

The involvement of TGF-β1 /FAK/α-SMA pathway in the antifibrotic impact of rice bran oi...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8716044
PloS One; Abdel-Rahman RF, Fayed HM et. al.

Dec 30th, 2021 - The objective of the current study is to investigate the effect of rice bran oil (RBO) on hepatic fibrosis as a characteristic response to persistent liver injuries. Rats were randomly allocated into five groups: the negative control group, thioacetamide (TAA) group (thioacetamide 100 mg/kg thrice weekly for two successive weeks, ip), RBO 0.2 and 0.4 groups (RBO 0.2mL and 0.4 mL/rat/day, po) an...

Multiplexed CRISPR-mediated engineering of protein secretory pathway genes in the therm...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8699913
PloS One; Kruasuwan W, Puseenam A et. al.

Dec 24th, 2021 - CRISPR multiplex gRNA systems have been employed in genome engineering in various industrially relevant yeast species. The thermotolerant methylotrophic yeast Ogataea thermomethanolica TBRC 656 is an alternative host for heterologous protein production. However, the limited secretory capability of this yeast is a bottleneck for protein production. Here, we refined CRISPR-based genome engineerin...

The Effect of CHIR 99021, a Glycogen Synthase Kinase-3β Inhibitor, on Transforming Grow...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8711002
Investigative Ophthalmology & Visual Science; Lee SY, Chae MK et. al.

Dec 24th, 2021 - This study investigated the effect of glycogen synthase kinase-3β (GSK-3β) inhibition on the fibrosis of human Tenon's fibroblasts (HTFs) induced by transforming growth factor-β (TGF-β). Quantitative real-time PCR and Western blot analyses were performed to determine the expression levels of molecules associated with the fibrosis of HTFs by TGF-β (fibronectin, collagen Iα, and α-smooth muscle a...

Metabolic engineering of Bacillus subtilis with an endopolygalacturonase gene isolated ...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8694468
PloS One; Rafique N, Bashir S et. al.

Dec 23rd, 2021 - Pectinolytic enzymes or pectinases are synthesized naturally by numerous microbes and plants. These enzymes degrade various kinds of pectin which exist as the major component of the cell wall in plants. A pectinase gene encoding endo-polygalacturonase (endo-PGase) enzyme was isolated from Pectobacterium carotovorum a plant pathogenic strain of bacteria and successfully cloned into a secretion v...

In vitro characterization of Haemonchus contortus trehalose-6-phosphate phosphatase and...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8685816
Parasites & Vectors; Wen Z, Xie X et. al.

Dec 22nd, 2021 - Trehalose-6-phosphate phosphatase (TPP6) is a key enzyme in the trehalose biosynthesis pathway. The accumulation of TPP6 inside the body is harmful to the pathogen, but almost nothing is currently known about the function of TPP6 from Haemonchus contortus (CRE-GOB-1). The H. contortus CRE-GOB-1 (HcGOB) gene was cloned and recombinant protein of GOB (rHcGOB) was expressed; transcription of the H...

A rare CTSC mutation in Papillon-Lefèvre Syndrome results in abolished serine protease ...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8691626
PloS One; Sanchez Klose FP, Björnsdottir H et. al.

Dec 22nd, 2021 - Papillon-Lefèvre Syndrome (PLS) is an autosomal recessive monogenic disease caused by loss-of-function mutations in the CTSC gene, thus preventing the synthesis of the protease Cathepsin C (CTSC) in a proteolytically active form. CTSC is responsible for the activation of the pro-forms of the neutrophil serine proteases (NSPs; Elastase, Proteinase 3 and Cathepsin G), suggesting its involvement i...

Relationship of Choroidal Vasculature and Choriocapillaris Flow With Alterations of Sal...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8709932
Investigative Ophthalmology & Visual Science; Scarinci F, Patacchioli FR et. al.

Dec 22nd, 2021 - An abnormality in choroidal vasculature is a known factor in the pathogenesis of central serous chorioretinopathy (CSC), a chorioretinal disease affecting mostly middle-aged males. The purpose of the present study was to investigate the role of the autonomic nervous system (ANS) in the pathophysiology of CSC. This was a cross-sectional observational study in which characteristic choroidal vascu...

KRSA: An R package and R Shiny web application for an end-to-end upstream kinase analys...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8682895
PloS One; DePasquale EAK, Alganem K et. al.

Dec 18th, 2021 - Phosphorylation by serine-threonine and tyrosine kinases is critical for determining protein function. Array-based platforms for measuring reporter peptide signal levels allow for differential phosphorylation analysis between conditions for distinct active kinases. Peptide array technologies like the PamStation12 from PamGene allow for generating high-throughput, multi-dimensional, and complex ...

WWP1 inactivation enhances efficacy of PI3K inhibitors while suppressing their toxiciti...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8670846
The Journal of Clinical Investigation; Kishikawa T, Higuchi H et. al.

Dec 16th, 2021 - Activation of the phosphatidylinositol 3-kinase (PI3K) signaling pathway is a pervasive event in tumorigenesis due to PI3K mutation and dysfunction of phosphatase and tensin homolog deleted on chromosome 10 (PTEN). Pharmacological inhibition of PI3K has resulted in variable clinical outcomes, however, raising questions regarding the possible mechanisms of unresponsiveness and resistance to trea...

Oxidative regulation of chloroplast enzymes by thioredoxin and thioredoxin-like protein...
https://doi.org/10.1073/pnas.2114952118
Proceedings of the National Academy of Sciences of the Un... Yokochi Y, Fukushi Y et. al.

Dec 16th, 2021 - Thioredoxin (Trx) is a protein that mediates the reducing power transfer from the photosynthetic electron transport system to target enzymes in chloroplasts and regulates their activities. Redox regulation governed by Trx is a system that is central to the adaptation of various chloroplast functions to the ever-changing light environment. However, the factors involved in the opposite reaction (...

Structure and RNA template requirements of Arabidopsis RNA-DEPENDENT RNA POLYMERASE 2.
https://doi.org/10.1073/pnas.2115899118
Proceedings of the National Academy of Sciences of the Un... Fukudome A, Singh J et. al.

Dec 15th, 2021 - RNA-dependent RNA polymerases play essential roles in RNA-mediated gene silencing in eukaryotes. In Arabidopsis, RNA-DEPENDENT RNA POLYMERASE 2 (RDR2) physically interacts with DNA-dependent NUCLEAR RNA POLYMERASE IV (Pol IV) and their activities are tightly coupled, with Pol IV transcriptional arrest, induced by the nontemplate DNA strand, somehow enabling RDR2 to engage Pol IV transcripts and...

Xanthatin Selectively Targets Retinoblastoma by Inhibiting the PLK1-Mediated Cell Cycle.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8684308
Investigative Ophthalmology & Visual Science; Yang J, Li Y et. al.

Dec 14th, 2021 - Retinoblastoma is the most common primary intraocular malignant tumor in children. Although intra-arterial chemotherapy and conventional chemotherapy have become promising therapeutic approaches for advanced intraocular retinoblastoma, the side effects threaten health and are unavoidable, making the development of targeted therapy an urgent need. Therefore, we intended to find a potential drug ...

Molecular basis of enzymatic nitrogen-nitrogen formation by a family of zinc-binding cu...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8664883
Nature Communications; Zhao G, Peng W et. al.

Dec 12th, 2021 - Molecules with a nitrogen-nitrogen (N-N) bond in their structures exhibit various biological activities and other unique properties. A few microbial proteins are recently emerging as dedicated N-N bond forming enzymes in natural product biosynthesis. However, the details of these biochemical processes remain largely unknown. Here, through in vitro biochemical characterization and computational ...

A secretory phospholipase D hydrolyzes phosphatidylcholine to suppress rice heading time.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8654219
PLoS Genetics; Qu L, Chu YJ et. al.

Dec 9th, 2021 - Phospholipase D (PLD) hydrolyzes membrane phospholipids and is crucial in various physiological processes and transduction of different signals. Secretory phospholipases play important roles in mammals, however, whose functions in plants remain largely unknown. We previously identified a rice secretory PLD (spPLD) that harbors a signal peptide and here we reported the secretion and function of ...

A D-2-hydroxyglutarate biosensor based on specific transcriptional regulator DhdR.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8651671
Nature Communications; Xiao D, Zhang W et. al.

Dec 9th, 2021 - D-2-Hydroxyglutarate (D-2-HG) is a metabolite involved in many physiological metabolic processes. When D-2-HG is aberrantly accumulated due to mutations in isocitrate dehydrogenase or D-2-HG dehydrogenase, it functions in a pro-oncogenic manner and is thus considered a therapeutic target and biomarker in many cancers. In this study, DhdR from Achromobacter denitrificans NBRC 15125 is identified...

Enhancing the production of physiologically active vitamin D3 by engineering the hydrox...
https://doi.org/10.1007/s11274-021-03193-1 10.1080/14786419.2013.872104 10.1006/abbi.2000.1970 10.1046/j.1523-1747.2001.01332.x 10.1002/bmb.2004.494032060394 10.1042/bst0100147 10.1146/annurev.bi.52.070183.002211 10.1016/j.bbrc.2009.05.033 10.1016/j.bbapap.2009.07.012 10.1016/j.bbagen.2006.07.017 10.1021/bi801222d 10.1016/j.bbrc.2016.03.139 10.1006/abio.2001.5214 10.1016/j.bbrc.2011.01.038 10.1073/pnas.88.20.8972 10.1002/med.2610070202 10.1007/s00449-015-1368-9 10.1007/BF02932307 10.1016/0167-4781(94)90266-6 10.1016/j.procbio.2017.07.030 10.1080/10242422.2016.1268130 10.1002/bit.24632 10.1146/annurev.nu.11.070191.001201 10.1016/j.bbapap.2010.07.014 10.1128/aem.57.10.2841-2846.1991 10.1016/j.bbrc.2004.05.140 10.1021/bi7023767
World Journal of Microbiology & Biotechnology; Fu B, Ren Q et. al.

Dec 9th, 2021 - In this study, the conversion of vitamin D3 (VD3) to its two active forms 25(OH)VD3 and 1α, 25(OH)2VD3 was carried out by engineering the hydroxylase CYP105A1 and its redox partners Fdx and Fdr. CYP105A1 and Fdx-Fdr were respectively expressed in E. coli BL21(DE3) and purified. The electron transport chain Fdx-Fdr had higher selectivity for the coenzyme NADH than NADPH. HPLC analysis showed tha...

Phenotypic effects from the expression of a deregulated AtGAD1 transgene and GABA pathw...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8648116
PloS One; S RM, Bedair MF et. al.

Dec 7th, 2021 - Glutamate decarboxylase (GAD; EC 4.1.1.15) catalyzes the irreversible decarboxylation of glutamate to produce γ-aminobutyric acid (GABA); a ubiquitous non-protein amino acid involved in the regulation of several aspects of plant metabolism and physiology. To study the function of GAD and GABA in maize, we have; 1) introduced native and deregulated forms of AtGAD1 into maize with the intent of i...

Prevalence of potential mediators of artemisinin resistance in African isolates of Plas...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8638531
Malaria Journal; Owoloye A, Olufemi M et. al.

Dec 4th, 2021 - The devastating public health impact of malaria has prompted the need for effective interventions. Malaria control gained traction after the introduction of artemisinin-based combination therapy (ACT). However, the emergence of artemisinin (ART) partial resistance in Southeast Asia and emerging reports of delayed parasite sensitivity to ACT in African parasites signal a gradual trend towards tr...

Paralog knockout profiling identifies DUSP4 and DUSP6 as a digenic dependence in MAPK p...
https://doi.org/10.1038/s41588-021-00967-z
Nature Genetics; Ito T, Young MJ et. al.

Dec 4th, 2021 - Although single-gene perturbation screens have revealed a number of new targets, vulnerabilities specific to frequently altered drivers have not been uncovered. An important question is whether the compensatory relationship between functionally redundant genes masks potential therapeutic targets in single-gene perturbation studies. To identify digenic dependencies, we developed a CRISPR paralog...