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About 363,968 results

A Microfluidic Platform for High-Purity Cell Free DNA Extraction from Plasma for Non-In...
https://doi.org/10.1002/pd.6092
Prenatal Diagnosis; Schneider L, Usherwood T et. al.

Jan 16th, 2022 - Increase the yield and purity of cell free DNA (cfDNA) extracted from plasma for non-invasive prenatal testing (NIPT) as inefficiencies in this extraction and purification can dramatically affect the sensitivity and specificity of the test. This work integrates cfDNA extraction from plasma with a microfluidic chip platform by combining magnetic bead-based extraction and electroosmotic flow on t...

Purification and characterization of a novel thermostable anticoagulant protein from me...
https://doi.org/10.1016/j.jep.2022.114990
Journal of Ethnopharmacology; Zhang Y, Yang R et. al.

Jan 16th, 2022 - The prevalence of cardiovascular disease (CVD) is increasing worldwide. Despite significant improvements in novel targeted treatment agents, natural products purified from medicinal animals with minimal side effects have attracted much attention. Several native proteins explored from suck-blood leeches, such as non-thermostable hirudin and its variants, revealed potent anticoagulant activity. T...

Neuronal subtype-specific growth cone and soma purification from mammalian CNS via frac...
https://doi.org/10.1038/s41596-021-00638-7
Nature Protocols; Engmann AK, Hatch JJ et. al.

Jan 14th, 2022 - During neuronal development, growth cones (GCs) of projection neurons navigate complex extracellular environments to reach distant targets, thereby generating extraordinarily complex circuitry. These dynamic structures located at the tips of axonal projections respond to substrate-bound as well as diffusible guidance cues in a neuronal subtype- and stage-specific manner to construct highly spec...

Comparative analysis of DNA extraction and PCR product purification methods for cervico...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8758110
PloS One; Shvartsman E, Richmond MEI et. al.

Jan 14th, 2022 - The microbiota of the lower female genital tract plays an important role in women's health. Microbial profiling using the chaperonin60 (cpn60) universal target (UT) improves resolution of vaginal species associated with negative health outcomes compared to the more commonly used 16S ribosomal DNA target. However, the choice of DNA extraction and PCR product purification methods may bias sequenc...

Co-infection of chickens with H9N2 and H7N9 avian influenza viruses leads to emergence ...
https://doi.org/10.1128/jvi.01856-21
Journal of Virology; Bhat S, James J et. al.

Jan 13th, 2022 - An H7N9 low pathogenicity avian influenza virus (LPAIV) emerged in 2013 through genetic reassortment between H9N2 and other LPAIVs circulating in birds in China. This virus causes inapparent clinical disease in chickens, but zoonotic transmission results in severe and fatal disease in humans. To examine a natural reassortment scenario between H7N9 and G1 lineage H9N2 viruses predominant in the ...

Fine pore engineering in a series of isoreticular metal-organic frameworks for efficien...
https://doi.org/10.1038/s41467-021-27929-7
Nature Communications; Wang J, Zhang Y et. al.

Jan 13th, 2022 - The separation of C2H2/CO2 is not only industrially important for acetylene purification but also scientifically challenging owing to their high similarities in physical properties and molecular sizes. Ultramicroporous metal-organic frameworks (MOFs) can exhibit a pore confinement effect to differentiate gas molecules of similar size. Herein, we report the fine-tuning of pore sizes in sub-nanom...

Leakage of Endotoxins through the Endotoxin Retentive Filter: An in vitro Study.
https://doi.org/10.1159/000520792
Blood Purification; Nozaki H, Tange Y et. al.

Jan 13th, 2022 - Ultrapurification of dialysis fluid has enabled highly efficient dialysis treatments. Online hemodiafiltration is one such treatment that uses a purified dialysis fluid as a supplemental fluid. In this method, an endotoxin retentive filter (ETRF) is used in the final step of dialysis fluid purification, with the aim of preventing leakage of endotoxins. Sodium hypochlorite and peracetic acid are...

Effect of Expanded Hemodialysis with Theranova® in Patients with COVID-19.
https://doi.org/10.1159/000520891
Blood Purification; Serrano Salazar ML, Portolés J et. al.

Jan 12th, 2022 - Cytokine storm control is the main target for improving severe COVID-19 by using immunosuppressive treatment. Effective renal replacement therapy (RRT) could give us an advantage removing cytokines in patients with RRT requirements superimposed on COVID-19. This is a prospective observational study in COVID-19 patients who required hemodialysis (HD). Patients were assigned to online hemodiafilt...

Helical structure motifs made searchable for functional peptide design.
https://doi.org/10.1038/s41467-021-27655-0
Nature Communications; Tsai CY, Salawu EO et. al.

Jan 12th, 2022 - The systematic design of functional peptides has technological and therapeutic applications. However, there is a need for pattern-based search engines that help locate desired functional motifs in primary sequences regardless of their evolutionary conservation. Existing databases such as The Protein Secondary Structure database (PSS) no longer serves the community, while the Dictionary of Prote...

Superoxide dismutase 3 prevents early stage diabetic retinopathy in streptozotocin-indu...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8751990
PloS One; Lee JY, Kim M et. al.

Jan 12th, 2022 - To identify the effects of superoxide dismutase (SOD)3 on diabetes mellitus (DM)-induced retinal changes in a diabetic rat model. Diabetic models were established by a single intraperitoneal injection of streptozotocin (STZ) in Sprague-Dawley rats. After purification of the recombinant SOD3, intravitreal injection of SOD3 was performed at the time of STZ injection, and 1 and 2 weeks following S...

Negative Ion Purifier Effects on Indoor Particulate Dosage to Small Airways.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8751072
International Journal of Environmental Research and Publi... Duan M, Wang L et. al.

Jan 12th, 2022 - Indoor air quality is an important health factor as we spend more than 80% of our time indoors. The primary type of indoor pollutant is particulate matter, high levels of which increase respiratory disease risk. Therefore, air purifiers are a common choice for addressing indoor air pollution. Compared with traditional filtration purifiers, negative ion air purifiers (NIAPs) have gained populari...

Conserved Proline Residues of Bacillus subtilis Intramembrane Metalloprotease SpoIVFB A...
https://doi.org/10.1128/JB.00386-21
Journal of Bacteriology; Olenic S, Buchanan F et. al.

Jan 11th, 2022 - Intramembrane metalloproteases regulate diverse biological processes by cleaving membrane-associated substrates within the membrane or near its surface. SpoIVFB is an intramembrane metalloprotease of Bacillus subtilis that cleaves Pro-σK during endosporulation. Intramembrane metalloproteases have a broadly conserved NPDG motif, which in the structure of an archaeal enzyme is located in a short ...

Epstein-Barr Virus BGLF2 commandeers RISC to interfere with cellular miRNA function.
https://doi.org/10.1371/journal.ppat.1010235
PLoS Pathogens; Campbell AM, De La Cruz Herrera CF et. al.

Jan 11th, 2022 - The Epstein-Barr virus (EBV) BGLF2 protein is a tegument protein with multiple effects on the cellular environment, including induction of SUMOylation of cellular proteins. Using affinity-purification coupled to mass-spectrometry, we identified the miRNA-Induced Silencing Complex (RISC), essential for miRNA function, as a top interactor of BGLF2. We confirmed BGLF2 interaction with the Ago2 and...

A Report on the First 7 Sequential Patients Treated Within the C-Reactive Protein Apher...
https://doi.org/10.12659/AJCR.935263
The American Journal of Case Reports; Schumann C, Heigl F et. al.

Jan 11th, 2022 - BACKGROUND Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-induced pneumonia is a disease with high mortality and, still, no effective treatment. Excessively elevated C-reactive protein (CRP) plasma levels inversely correlate with prognosis. As CRP, via complement and macrophage activation, can cause organ damage in COVID-19, we have recently introduced selective CRP apheresis as a...

Application of cellulosic materials as supports for single-step purification and immobi...
https://doi.org/10.1016/j.ijbiomac.2022.01.006
International Journal of Biological Macromolecules; Gennari A, Simon R et. al.

Jan 11th, 2022 - This study aimed to develop single-step purification and immobilization processes on cellulosic supports of β-galactosidase from Kluyveromyces sp. combined with the Cellulose-Binding Domain (CBD) tag. After 15 min of immobilization, with an enzymatic load of 150 U/gsupport, expressed activity values reached 106.88 (microcrystalline cellulose), 115.03 (alkaline nanocellulose), and 108.47 IU/g (a...

Purification, structural characterization and neuroprotective effect of a neutral polys...
https://doi.org/10.1016/j.ijbiomac.2021.12.165
International Journal of Biological Macromolecules; Zhang W, Hu B et. al.

Jan 10th, 2022 - In the present study, a purified polysaccharide (named SCP-1, Mw 1.368 × 104 Da) was isolated from Sparassis crispa, and its biological activity was evaluated in an oxidative stress model caused by H2O2 in hippocampal neuronal HT22 cells. SCP-1 was a heteropolysaccharide mainly comprising glucose, galactose, fucose, and mannose in a molar ratio of 52.10: 31.10: 15.04: 1.76. The main backbone of...

Ononin: A candidate anti-parasitic drug isolated from Spatholobi caulis against infecti...
https://doi.org/10.1016/j.parint.2021.102535
Parasitology International; Yang Z, Tan X et. al.

Jan 8th, 2022 - Dactylogyrus is a common parasitic pathogen, which causes high mortality of fish when presents in large numbers, resulting in serious economic losses. Herbal medicines contain myriad of bioactive compounds is a valuable reserve for developing safe and effective anti-parasite drugs. Here, we conducted bioassay-guided fractionation to isolate and identify the anti-parasitic constituents from Spat...

Isolation, structures and bioactivities of the polysaccharides from Radix Hedysari: A r...
https://doi.org/10.1016/j.ijbiomac.2021.12.095
International Journal of Biological Macromolecules; Mo X, Guo D et. al.

Jan 8th, 2022 - Radix Hedysari, a well-known traditional Chinese herbal medicine, has a long history as a medicinal plant in China based on its wide spectrum of biological and pharmacological activities. Until now, many chemical constituents have been isolated and identified from Radix Hedysari, such as polysaccharides, flavonoids, phenylpropanoids, trace elements and so on. Of these, Radix Hedysari polysaccha...

The First Lady Presses for Cancer Screening to Get Back On Track.
https://doi.org/10.1158/1940-6207.CAPR-21-0548
Cancer Prevention Research (Philadelphia, Pa.); DuBois LA, DuBois RN

Jan 8th, 2022 - The First Lady of the United States, Dr. Jill Biden, visited the Hollings Cancer Center at the Medical University of South Carolina on October 25, 2021. This Commentary remarks on the administration's goal of directing public attention to cancer screening and prevention as part of an overall effort to recover ground lost in the COVID-19 pandemic, particularly in underserved communities.

Obesity as a mortality risk factor in the medical ward: a case control study.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8733434
BMC Endocrine Disorders; Soffer S, Zimlichman E et. al.

Jan 8th, 2022 - Research regarding the association between severe obesity and in-hospital mortality is inconsistent. We evaluated the impact of body mass index (BMI) levels on mortality in the medical wards. The analysis was performed separately before and during the COVID-19 pandemic. We retrospectively retrieved data of adult patients admitted to the medical wards at the Mount Sinai Health System in New York...