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About 103,255 results

Energy metabolism of cells in the macula flava of the newborn vocal fold from the aspec...
https://doi.org/10.1017/S0022215121001614
The Journal of Laryngology and Otology; Sato K, Chitose S et. al.

Aug 28th, 2021 - Cells in the vocal fold of maculae flavae are likely to be tissue stem cells. Energy metabolism of the cells in newborn maculae flavae was investigated from the aspect of mitochondrial microstructure. Five normal newborn vocal folds were investigated under transmission electron microscopy. Mitochondria consisted of a double membrane bounded body containing matrices and a system of cristae. Howe...

The antidepressant drug vilazodone is an allosteric inhibitor of the serotonin transpor...
https://doi.org/10.1038/s41467-021-25363-3 10.1038/nrn3708 10.1016/j.str.2013.03.002 10.1146/annurev.med.60.042307.110802 10.1016/j.tips.2006.05.003 10.1124/pr.108.000869 10.1016/bs.mie.2015.01.002 10.1093/nar/gkj001 10.1038/nature03978 10.1038/nature10737 10.1038/nsmb.2816 10.1038/nsmb.2894 10.1016/j.bpj.2015.02.010 10.1126/sciadv.aar6179 10.1038/s41467-017-02202-y 10.1016/j.bbagen.2014.04.011 10.1038/211969a0 10.1038/s41467-019-10449-w 10.1146/annurev-biochem-060815-014520 10.1038/s41586-019-1135-1 10.1146/annurev.ne.16.030193.000445 10.1074/jbc.M111.304261 10.1038/ncomms12755 10.1038/354066a0 10.1126/science.1948036 10.1177/0269881115609072 10.1377/hlthaff.2010.1024 10.4068/cmj.2018.54.2.101 10.1016/j.molcel.2008.05.008 10.1038/nature12533 10.1074/jbc.M112.371765 10.1038/nature06038 10.1002/anie.201508755 10.1016/j.ddtec.2012.07.007 10.1074/jbc.R115.662759 10.1021/acschemneuro.9b00262 10.1038/s41467-020-15292-y 10.1371/journal.pone.0200085 10.1016/j.tips.2020.04.006 10.1038/nature17629 10.1016/j.euroneuro.2004.08.008 10.1016/j.ejphar.2005.01.018 10.4068/cmj.2016.52.2.91 10.1017/S1092852915000139 10.1097/JCP.0b013e31822c6741 10.4103/IJPSYM.IJPSYM_368_17 10.1097/YIC.0000000000000057 10.4088/JCP.v61n0406 10.1039/C9CP05764A 10.1021/jm040793q 10.1074/jbc.M109.045401 10.1038/ncomms11261 10.1074/jbc.M112.342212 10.1074/jbc.273.31.19459 10.1074/jbc.M505055200 10.1074/jbc.M405579200 10.1111/j.1471-4159.2004.02835.x 10.1111/bph.13411 10.1016/S0014-2999(00)00565-3 10.1007/BF00537461 10.1038/2041318a0 10.1038/s41594-018-0026-8 10.1021/acs.jmedchem.9b01105 10.1021/acsptsci.8b00060 10.1073/pnas.0600905103 10.1124/mol.107.039800 10.1016/j.biopsych.2012.03.022 10.1038/s41386-019-0366-z 10.1080/10401230490453743 10.1038/nprot.2014.173 10.1016/j.jsb.2005.07.007 10.1016/j.str.2017.06.017 10.1016/j.jsb.2015.11.003 10.1016/j.jsb.2009.01.004 10.1038/nmeth.4169 10.1016/j.jmb.2003.07.013 10.1002/jcc.20084 10.1107/S0907444904019158 10.1107/S0907444909052925 10.1107/S0907444909042073 10.1007/s10822-007-9133-z 10.1093/nar/gkr703 10.1021/acs.jctc.5b00935 10.1038/nmeth.4067 10.1021/jp101759q 10.1063/1.445869 10.1103/PhysRevA.31.1695 10.1080/00268978300102851 10.1063/1.464397 10.1002/(SICI)1096-987X(199709)18:12<1463::AID-JCC4>3.0.CO;2-H 10.1016/0263-7855(96)00018-5 10.1021/ci500020m
Nature Communications; Plenge P, Yang D et. al.

Aug 22nd, 2021 - Depression is a common mental disorder. The standard medical treatment is the selective serotonin reuptake inhibitors (SSRIs). All characterized SSRIs are competitive inhibitors of the serotonin transporter (SERT). A non-competitive inhibitor may produce a more favorable therapeutic profile. Vilazodone is an antidepressant with limited information on its molecular interactions with SERT. Here w...

Molecular recognition of an acyl-peptide hormone and activation of ghrelin receptor.
https://doi.org/10.1038/s41467-021-25364-2 10.1016/S1043-2760(00)00362-3 10.1038/45230 10.1016/j.cmet.2018.02.008 10.1111/j.1467-789X.2006.00270.x 10.1016/j.cell.2008.01.017 10.1073/pnas.0800708105 10.1210/jc.2004-1641 10.1002/dta.1947 10.1002/dta.1787 10.1126/science.273.5277.974 10.1016/j.cmet.2017.10.016 10.1016/j.tips.2004.01.010 10.1210/mend.12.1.0051 10.1016/bs.pmbts.2016.02.001 10.1124/mol.108.049189 10.1074/jbc.M609796200 10.1016/j.str.2018.12.004 10.1038/s41467-020-17554-1 10.1073/pnas.1905105116 10.1074/jbc.M407676200 10.1074/jbc.M112.383240 10.1016/j.ejphar.2010.06.018 10.1038/387206a0 10.1016/j.str.2012.01.019 10.1038/s41467-020-17933-8 10.1016/j.cell.2020.08.024 10.1126/science.aaw5188 10.1038/nature10361 10.1006/bbrc.2001.5553 10.1517/13543784.6.12.1839 10.1038/s41586-018-0219-7 10.1038/nmeth.4193 10.1016/j.jsb.2015.08.008 10.1016/j.ab.2010.09.030
Nature Communications; Wang Y, Guo S et. al.

Aug 22nd, 2021 - Ghrelin, also called "the hunger hormone", is a gastric peptide hormone that regulates food intake, body weight, as well as taste sensation, reward, cognition, learning and memory. One unique feature of ghrelin is its acylation, primarily with an octanoic acid, which is essential for its binding and activation of the ghrelin receptor, a G protein-coupled receptor. The multifaceted roles of ghre...

In situ ultrastructures of two evolutionarily distant apicomplexan rhoptry secretion sy...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371170
Nature Communications; Mageswaran SK, Guérin A et. al.

Aug 19th, 2021 - Parasites of the phylum Apicomplexa cause important diseases including malaria, cryptosporidiosis and toxoplasmosis. These intracellular pathogens inject the contents of an essential organelle, the rhoptry, into host cells to facilitate invasion and infection. However, the structure and mechanism of this eukaryotic secretion system remain elusive. Here, using cryo-electron tomography and subtom...

Structures of tmRNA and SmpB as they transit through the ribosome.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8363625
Nature Communications; Guyomar C, D'Urso G et. al.

Aug 15th, 2021 - In bacteria, trans-translation is the main rescue system, freeing ribosomes stalled on defective messenger RNAs. This mechanism is driven by small protein B (SmpB) and transfer-messenger RNA (tmRNA), a hybrid RNA known to have both a tRNA-like and an mRNA-like domain. Here we present four cryo-EM structures of the ribosome during trans-translation at resolutions from 3.0 to 3.4 Å. These include...

Genomic characterization of four novel bacteriophages infecting the clinical pathogen K...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8386662
DNA Research : an International Journal for Rapid Publica... Bonilla E, Costa AR et. al.

Aug 15th, 2021 - Bacteriophages are an invaluable source of novel genetic diversity. Sequencing of phage genomes can reveal new proteins with potential uses as biotechnological and medical tools, and help unravel the diversity of biological mechanisms employed by phages to take over the host during viral infection. Aiming to expand the available collection of phage genomes, we have isolated, sequenced, and asse...

Patched regulates lipid homeostasis by controlling cellular cholesterol levels.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8361143
Nature Communications; Cadena Del Castillo CE, Hannich JT et. al.

Aug 14th, 2021 - Hedgehog (Hh) signaling is essential during development and in organ physiology. In the canonical pathway, Hh binding to Patched (PTCH) relieves the inhibition of Smoothened (SMO). Yet, PTCH may also perform SMO-independent functions. While the PTCH homolog PTC-3 is essential in C. elegans, worms lack SMO, providing an excellent model to probe non-canonical PTCH function. Here, we show that PTC...

Cryo-EM structures of the TTYH family reveal a novel architecture for lipid interactions.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8361169
Nature Communications; Sukalskaia A, Straub MS et. al.

Aug 14th, 2021 - The Tweety homologs (TTYHs) are members of a conserved family of eukaryotic membrane proteins that are abundant in the brain. The three human paralogs were assigned to function as anion channels that are either activated by Ca2+ or cell swelling. To uncover their unknown architecture and its relationship to function, we have determined the structures of human TTYH1-3 by cryo-electron microscopy...

Rational design of pyrrole derivatives with aggregation-induced phosphorescence charact...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8361132
Nature Communications; Yang J, Zhang Y et. al.

Aug 14th, 2021 - Pure organic room-temperature phosphorescent (RTP) materials have been suggested to be promising bioimaging materials due to their good biocompatibility and long emission lifetime. Herein, we report a class of RTP materials. These materials are developed through the simple introduction of an aromatic carbonyl to a tetraphenylpyrrole molecule and also exhibit aggregation-induced emission (AIE) p...

Protein interface redesign facilitates the transformation of nanocage building blocks t...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8357837
Nature Communications; Zhang X, Liu Y et. al.

Aug 13th, 2021 - Although various artificial protein nanoarchitectures have been constructed, controlling the transformation between different protein assemblies has largely been unexplored. Here, we describe an approach to realize the self-assembly transformation of dimeric building blocks by adjusting their geometric arrangement. Thermotoga maritima ferritin (TmFtn) naturally occurs as a dimer; twelve of thes...

Polyclonal antibody responses to HIV Env immunogens resolved using cryoEM.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8355326
Nature Communications; Antanasijevic A, Sewall LM et. al.

Aug 12th, 2021 - Engineered ectodomain trimer immunogens based on BG505 envelope glycoprotein are widely utilized as components of HIV vaccine development platforms. In this study, we used rhesus macaques to evaluate the immunogenicity of several stabilized BG505 SOSIP constructs both as free trimers and presented on a nanoparticle. We applied a cryoEM-based method for high-resolution mapping of polyclonal anti...

The structural dynamics of macropinosome formation and PI3-kinase-mediated sealing reve...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8355319
Nature Communications; Quinn SE, Huang L et. al.

Aug 12th, 2021 - Macropinosomes are formed by shaping actin-rich plasma membrane ruffles into large intracellular organelles in a phosphatidylinositol 3-kinase (PI3K)-coordinated manner. Here, we utilize lattice lightsheet microscopy and image visualization methods to map the three-dimensional structure and dynamics of macropinosome formation relative to PI3K activity. We show that multiple ruffling morphologie...

A new dicistro-like virus from soldier fly, Inopus flavus (Diptera: Stratiomyidae), a p...
https://doi.org/10.1007/s00705-021-05171-2 10.1071/ZO9880627 10.1603/0022-0493-94.5.1097 10.1111/1744-7917.12676 10.1099/0022-1317-69-7-1617 10.1099/jgv.0.000756 10.1186/1743-422X-6-193 10.1093/nar/gky1121 10.1099/vir.0.79992-0 10.1159/000149886 10.1159/000149489 10.1099/0022-1317-69-4-787 10.1016/0042-6822(81)90275-0 10.1006/jipa.1997.4721 10.1099/0022-1317-73-4-1021 10.1016/j.virol.2004.07.016 10.1111/j.1744-7348.1985.tb03136.x 10.1099/vir.0.83284-0 10.1099/vir.0.000068 10.1111/j.1570-7458.2008.00675.x 10.1146/annurev-ento-112408-085457 10.1371/journal.ppat.1001005 10.1261/rna.5208104 10.1016/j.virusres.2005.10.011 10.1128/JVI.74.11.5123-5132.2000 10.1099/0022-1317-34-2-269
Archives of Virology; Asselin AK, Etebari K et. al.

Aug 7th, 2021 - Native Australian soldier flies, Inopus spp. (Diptera: Stratiomyidae), are agricultural pests of economic importance to the sugarcane industry. A screen of the salivary gland transcriptome of Inopus flavus (James) revealed the presence of viral RNA belonging to a potentially novel member of the family Dicistroviridae. The complete genome sequence consists of 9793 nucleotides with two open readi...

Connectomes across development reveal principles of brain maturation.
https://doi.org/10.1038/s41586-021-03778-8 10.1523/JNEUROSCI.3679-04.2005 10.1016/0300-9629(94)90308-5 10.7554/eLife.29089 10.7554/eLife.42135 10.1038/scientificamerican0979-150 10.1016/j.conb.2018.10.002 10.1098/rstb.1986.0056 10.7554/eLife.57443 10.7554/eLife.46876 10.1016/j.neuron.2020.02.001 10.7554/eLife.08069 10.1093/comnet/cnu026 10.1016/j.cell.2016.01.007 10.1038/271764a0 10.1038/nmeth.2451 10.1371/journal.pcbi.1001066 10.1093/genetics/77.1.71 10.3389/fncir.2018.00094 10.1016/0012-1606(77)90158-0 10.1016/bs.mcb.2019.04.004 10.3389/fncir.2014.00068 10.1371/journal.pone.0038011 10.1038/nmeth.2072 10.1093/bioinformatics/btp266 10.7554/eLife.19887 10.1038/emboj.2013.91 10.1016/j.cell.2015.06.054 10.1006/jpdc.1996.0107 10.3389/fncir.2018.00088 10.7554/eLife.35692 10.1002/cne.901630207 10.1523/JNEUROSCI.19-01-00072.1999 10.1038/s41586-019-1352-7 10.1038/s41598-018-31202-1
Nature Witvliet D, Mulcahy B et. al.

Aug 6th, 2021 - An animal's nervous system changes as its body grows from birth to adulthood and its behaviours mature1-8. The form and extent of circuit remodelling across the connectome is unknown3,9-15. Here we used serial-section electron microscopy to reconstruct the full brain of eight isogenic Caenorhabditis elegans individuals across postnatal stages to investigate how it changes with age. The overall ...

Structural insights into how Prp5 proofreads the pre-mRNA branch site.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8357632
Nature Zhang Z, Rigo N et. al.

Aug 6th, 2021 - During the splicing of introns from precursor messenger RNAs (pre-mRNAs), the U2 small nuclear ribonucleoprotein (snRNP) must undergo stable integration into the spliceosomal A complex-a poorly understood, multistep process that is facilitated by the DEAD-box helicase Prp5 (refs. 1-4). During this process, the U2 small nuclear RNA (snRNA) forms an RNA duplex with the pre-mRNA branch site (the U...

Variant PNLDC1, Defective piRNA Processing, and Azoospermia.
https://doi.org/10.1056/NEJMoa2028973
The New England Journal of Medicine; Nagirnaja L, Mørup N et. al.

Aug 5th, 2021 - P-element-induced wimpy testis (PIWI)-interacting RNAs (piRNAs) are short (21 to 35 nucleotides in length) and noncoding and are found almost exclusively in germ cells, where they regulate aberrant expression of transposable elements and postmeiotic gene expression. Critical to the processing of piRNAs is the protein poly(A)-specific RNase-like domain containing 1 (PNLDC1), which trims their 3'...

Characterization of the first Pseudomonas grimontii bacteriophage, PMBT3.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8421299
Archives of Virology; Sprotte S, Brinks E et. al.

Aug 5th, 2021 - The complete genome sequence of the virulent bacteriophage PMBT3, isolated on the proteolytic Pseudomonas grimontii strain MBTL2-21, showed no significant similarity to other known phage genome sequences, making this phage the first reported to infect a strain of P. grimontii. Electron microscopy revealed PMBT3 to be a member of the family Siphoviridae, with notably long and flexible whiskers. ...

Quantifying nanodiamonds biodistribution in whole cells with correlative iono-nanoscopy.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8329174
Nature Communications; Mi Z, Chen CB et. al.

Aug 4th, 2021 - Correlative imaging and quantification of intracellular nanoparticles with the underlying ultrastructure is crucial for understanding cell-nanoparticle interactions in biological research. However, correlative nanoscale imaging of whole cells still remains a daunting challenge. Here, we report a straightforward nanoscopic approach for whole-cell correlative imaging, by simultaneous ionoluminesc...

Correlative multi-scale cryo-imaging unveils SARS-CoV-2 assembly and egress.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8324836
Nature Communications; Mendonça L, Howe A et. al.

Aug 1st, 2021 - Since the outbreak of the SARS-CoV-2 pandemic, there have been intense structural studies on purified viral components and inactivated viruses. However, structural and ultrastructural evidence on how the SARS-CoV-2 infection progresses in the native cellular context is scarce, and there is a lack of comprehensive knowledge on the SARS-CoV-2 replicative cycle. To correlate cytopathic events indu...

A synthetic nanobody targeting RBD protects hamsters from SARS-CoV-2 infection.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8324831
Nature Communications; Li T, Cai H et. al.

Aug 1st, 2021 - SARS-CoV-2, the causative agent of COVID-191, features a receptor-binding domain (RBD) for binding to the host cell ACE2 protein1-6. Neutralizing antibodies that block RBD-ACE2 interaction are candidates for the development of targeted therapeutics7-17. Llama-derived single-domain antibodies (nanobodies, ~15 kDa) offer advantages in bioavailability, amenability, and production and storage owing...