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About 106,275 results

Perspective - ultrastructural analyses reflect the effects of sleep and sleep loss on n...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9113019
Sleep Wang L, Aton SJ

May 14th, 2022 - Recent electron microscopic analyses of neurons in the Drosophila and rodent brain demonstrate that acute or chronic sleep loss can alter the structures of various organelles, including mitochondria, nucleus, and Golgi apparatus. Here, we discuss these findings in the context of biochemical findings from the sleep deprived brain, to clarify how these morphological changes may related to altered...

Atomic Structures of Coxsackievirus B5 Provide Key Information on Viral Evolution and S...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9093117
Journal of Virology; Yang P, Shi D et. al.

Apr 21st, 2022 - Coxsackie virus B5 (CVB5), a main serotype in human Enterovirus B (EVB), can cause severe viral encephalitis and aseptic meningitis among infants and children. Currently, there is no approved vaccine or antiviral therapy available against CVB5 infection. Here, we determined the atomic structures of CVB5 in three forms: mature full (F) particle (2.73 Å), intermediate altered (A) particle (2.81 Å...

Atomic Structure of the Human Sapovirus Capsid Reveals a Unique Capsid Protein Conforma...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9093105
Journal of Virology; Miyazaki N, Song C et. al.

Apr 19th, 2022 - Sapovirus (SaV) is a member of the Caliciviridae family, which causes acute gastroenteritis in humans and animals. Human sapoviruses (HuSaVs) are genetically and antigenically diverse, but the lack of a viral replication system and structural information has hampered the development of vaccines and therapeutics. Here, we successfully produced a self-assembled virus-like particle (VLP) from the ...

Structure of active human telomerase with telomere shelterin protein TPP1.
https://doi.org/10.1038/s41586-022-04582-8 10.1101/cshperspect.a003558 10.1016/j.cell.2020.12.028 10.3892/ol.2020.11659 10.1038/s41580-020-0234-z 10.1158/2159-8290.CD-16-0062 10.1016/j.csbj.2020.06.040 10.1146/annurev-genet-032918-021921 10.1038/s41586-021-03415-4 10.1038/s41586-018-0062-x 10.1038/s41586-021-03529-9 10.1016/j.str.2015.08.006 10.1074/jbc.M201266200 10.1093/nar/gkm700 10.1083/jcb.201401012 10.1146/annurev-biochem-061516-045019 10.1101/gad.263863.115 10.1074/jbc.REV120.014017 10.1182/blood.2019003264 10.1182/blood.2021011075 10.1016/j.cell.2018.04.038 10.1126/science.aab4070 10.1038/s41580-021-00328-y 10.1038/nsmb867 10.1038/nature11648 10.1016/j.cell.2012.07.012 10.1074/jbc.M112.394767 10.1016/j.celrep.2018.01.012 10.1038/ncomms14929 10.1038/ncomms14928 10.1016/j.cell.2016.07.033 10.1128/MCB.19.1.567 10.1073/pnas.1100279108 10.1038/nature05454 10.7554/eLife.03563 10.1073/pnas.1911912116 10.1073/pnas.2011684117 10.1128/MCB.00792-13 10.1038/sj.embor.7400374 10.1038/nsmb.3101 10.1101/gad.1140303 10.1038/s41422-021-00586-7 10.1016/j.molcel.2005.01.017 10.1016/j.jmb.2008.10.005 10.1073/pnas.1013269107 10.1261/rna.077255.120 10.1038/nsmb.2819 10.1038/s41388-020-01405-w 10.1101/gad.1476206 10.1093/nar/25.23.4740 10.1016/S1097-2765(03)00196-5 10.1038/26912 10.1007/978-1-60327-475-3_2 10.1016/j.jsb.2005.07.007 10.7554/eLife.42166 10.1038/nmeth.4193 10.1016/j.jsb.2015.08.008 10.1038/s41592-019-0575-8 10.1016/j.jmb.2003.07.013 10.1107/S0907444910007493 10.1002/jcc.20084 10.1107/S0907444909052925 10.1002/pro.3235 10.1073/pnas.0407141102 10.1073/pnas.1520420113 10.1038/emboj.2009.409 10.1073/pnas.1814777116 10.1093/nar/gkf481 10.1093/nar/gku316 10.1182/blood-2018-03-837799 10.1183/13993003.00809-2019
Nature Liu B, He Y et. al.

Apr 15th, 2022 - Human telomerase is a RNA-protein complex that extends the 3' end of linear chromosomes by synthesizing multiple copies of the telomeric repeat TTAGGG1. Its activity is a determinant of cancer progression, stem cell renewal and cellular aging2-5. Telomerase is recruited to telomeres and activated for telomere repeat synthesis by the telomere shelterin protein TPP16,7. Human telomerase has a bil...

Complete genome analysis of the novel Shewanella phage vB_Sb_QDWS.
https://doi.org/10.1007/s00705-022-05435-5 10.3389/fmicb.2019.00135 10.1016/j.fm.2021.103755 10.1111/lam.12687 10.1016/j.foodchem.2018.02.069 10.1007/s00203-020-02167-5 10.1007/s13197-015-1996-8 10.1007/s00705-015-2647-0 10.1016/S0021-9258(19)49724-X 10.1128/AEM.02279-13 10.1093/nar/gki366 10.1093/bib/bbn017
Archives of Virology; Tan L, Xuan G et. al.

Apr 9th, 2022 - We present here the results of the analysis of the complete genome sequence of a potentially temperate phage, vB_Sb_QDWS, which was isolated from wastewater samples collected in Qingdao, China. The genome of phage vB_Sb_QDWS is composed of a double-stranded DNA that is 47,902 bp in length with a G + C content of 63.16%. It is predicted to contain 69 putative protein-encoding genes. Microscopic ...

Chemical-genetic interaction mapping links carbon metabolism and cell wall structure to...
https://doi.org/10.1073/pnas.2201632119
Proceedings of the National Academy of Sciences of the Un... Koh EI, Oluoch PO et. al.

Apr 6th, 2022 - Current chemotherapy against Mycobacterium tuberculosis (Mtb), an important human pathogen, requires a multidrug regimen lasting several months. While efforts have been made to optimize therapy by exploiting drug–drug synergies, testing new drug combinations in relevant host environments remains arduous. In particular, host environments profoundly affect the bacterial metabolic state and drug e...

Differential assembly diversifies GABAA receptor structures and signalling.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7612593
Nature Sente A, Desai R et. al.

Apr 1st, 2022 - Type A γ-aminobutyric acid receptors (GABAARs) are pentameric ligand-gated chloride channels that mediate fast inhibitory signalling in neural circuits1,2 and can be modulated by essential medicines including general anaesthetics and benzodiazepines3. Human GABAAR subunits are encoded by 19 paralogous genes that can, in theory, give rise to 495,235 receptor types. However, the principles that g...

Lipogenic stromal cells as members of the foam-cell population in human atherosclerosis...
https://doi.org/10.1080/01913123.2022.2059042
Ultrastructural Pathology; Ru YX, Xue-Bin Z et. al.

Mar 31st, 2022 - To identify the nature of foam cells in atherosclerosis, carotid atherosclerotic plaques (CAPs) from six patients were studied. Hematoxylin-and-eosin, Congo Red and Oil Red O staining were used to study histopathologic alterations in CAPs. CD31, α-smooth-muscle actin (α-SMA), CD68, desmin and S100 were stained immunohistochemically. The ultrastructure of foam cells was analyzed by transmission ...

Particle Morphology of Medusavirus Inside and Outside the Cells Reveals a New Maturatio...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9006890
Journal of Virology; Watanabe R, Song C et. al.

Mar 18th, 2022 - Medusavirus, a giant virus, is phylogenetically closer to eukaryotes than the other giant viruses and has been recently classified as an independent species. However, details of its morphology and maturation process in host cells remain unclear. Here, we investigated the particle morphology of medusavirus inside and outside infected cells using conventional transmission electron microscopy (C-T...

Microglia regulate chandelier cell axo-axonic synaptogenesis.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8931231
Proceedings of the National Academy of Sciences of the Un... Gallo NB, Berisha A et. al.

Mar 10th, 2022 - SignificanceChandelier cells (ChCs) are a unique type of GABAergic interneuron that form axo-axonic synapses exclusively on the axon initial segment (AIS) of neocortical pyramidal neurons (PyNs), allowing them to exert powerful yet precise control over PyN firing and population output. The importance of proper ChC function is further underscored by the association of ChC connectivity defects wi...

FliL ring enhances the function of periplasmic flagella.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8931381
Proceedings of the National Academy of Sciences of the Un... Guo S, Xu H et. al.

Mar 8th, 2022 - SignificanceHow flagella sense complex environments and control bacterial motility remain fascinating questions. Here, we deploy cryo-electron tomography to determine in situ structures of the flagellar motor in wild-type and mutant cells of Borrelia burgdorferi, revealing that three flagellar proteins (FliL, MotA, and MotB) form a unique supramolecular complex in situ. Importantly, FliL not on...

CD38 Expression by Circulating and Skin-Infiltrating Lymphocytes from Sezary Syndrome P...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8896155
Disease Markers; Quaglino P, Novelli M et. al.

Mar 8th, 2022 - Reports on the expression of CD38 in Sézary syndrome (SS), erythrodermic primary cutaneous T cell lymphoma with leukemic involvement, are limited. The aim of the present study is the analysis of the expression of CD38 by skin-infiltrating mononuclear cells and circulating T lymphocytes in a cohort of SS patients. SS patients diagnosed since 1985 in our clinic were retrospectively analyzed for C...

Evolution of the SARS-CoV-2 spike protein in the human host.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8897445
Nature Communications; Wrobel AG, Benton DJ et. al.

Mar 6th, 2022 - Recently emerged variants of SARS-CoV-2 contain in their surface spike glycoproteins multiple substitutions associated with increased transmission and resistance to neutralising antibodies. We have examined the structure and receptor binding properties of spike proteins from the B.1.1.7 (Alpha) and B.1.351 (Beta) variants to better understand the evolution of the virus in humans. Spikes of both...

Cryo-EM structure of a SARS-CoV-2 omicron spike protein ectodomain.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8894419
Nature Communications; Ye G, Liu B et. al.

Mar 5th, 2022 - The omicron variant of SARS-CoV-2 has been spreading rapidly across the globe. The virus-surface spike protein plays a critical role in the cell entry and immune evasion of SARS-CoV-2. Here we determined the 3.0 Å cryo-EM structure of the omicron spike protein ectodomain. In contrast to the original strain of SARS-CoV-2 where the receptor-binding domain (RBD) of the spike protein takes a mixtur...

Melanocytes and keratinocytes morphological changes in vitiligo patients. A histologica...
https://doi.org/10.1080/01913123.2022.2044946
Ultrastructural Pathology; Elsherif R, Mahmoud WA et. al.

Mar 5th, 2022 - Vitiligo is an idiopathic acquired chronic stigmatizing disease. It is a pigmentary disorder that affects the skin and the mucous membranes, and it is characterized by well-circumscribed, depigmented milky white macules and patches. It has an estimated prevalence of 0.5-2% of the population worldwide. In the previous studies, several mechanisms such as autoimmune, oxidative stress, genetic fact...

Homozygous SPAG6 variants can induce nonsyndromic asthenoteratozoospermia with severe M...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8886842
Reproductive Biology and Endocrinology : RB&E; Xu C, Tang D et. al.

Mar 3rd, 2022 - Multiple morphological abnormalities of the sperm flagella (MMAF) is a subtype of severe asthenoteratozoospermia with poorly understood genetic etiology. SPAG6 is a core axonemal component that plays a critical role in the formation of cilia and sperm flagella. Previous studies have reported that mutations in SPAG6 cause primary ciliary dyskinesia (PCD), but the association between SPAG6 gene v...

Comparison of bone microstructures via high-resolution peripheral quantitative computed...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8890516
Renal Failure; Tsuji K, Kitamura M et. al.

Mar 1st, 2022 - Chronic kidney disease (CKD) negatively affects bone strength; however, the osteoporotic conditions in patients with CKD are not fully understood. Moreover, the changes in bone microstructure between pre-dialysis and dialysis are unknown. High-resolution peripheral quantitative computed tomography (HR-pQCT) reveals the three-dimensional microstructures of the bone. We aimed to evaluate bone mic...

Adhesion properties of cell surface proteins in Lactobacillus strains in the GIT enviro...
https://doi.org/10.1039/d1fo04328e
Food & Function; Yu Y, Zong M et. al.

Mar 1st, 2022 - As a broadly defined member of lactic acid bacteria (LAB), the Lactobacillus strain is well characterized in food fermentation and specific strains can enhance the intestinal barrier function and be recognized as the probiotic strain. In recent years, many molecules of the cell surface are thought to be related to the adhesion property in the gastrointestinal mucosa. Mucus layer-related protein...

Proteonematalycus wagneri Kethley reveals where the opisthosoma begins in acariform mites.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8880937
PloS One; Bolton SJ

Feb 26th, 2022 - It is generally thought that the anterior border of the opisthosoma of acariform mites is delineated by the disjugal furrow, but there is no evidence to support this other than the superficial appearance of tagmosis in some oribatids. It is proposed herein that the disjugal furrow is an apomorphic feature that does not correspond with any segmental borders. Although the disjugal furrow is absen...

Significance of eggshell morphology as an additional tool to distinguish species of san...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8880748
PloS One; Jariyapan N, Tippawangkosol P et. al.

Feb 26th, 2022 - Morphological characteristics of eggshells are important in sand fly ootaxonomy. In this study, eggshells from Phlebotomus stantoni Newstead, Sergentomyia khawi (Raynal), and Grassomyia indica (Theodor) sand flies collected in Chiang Mai province, Thailand were examined and characterized using light microscopy (LM) and scanning electron microscopy (SEM). Then, eggshell morphology of these three...